skip to main content
Idioma:
Tipo de recurso Mostra resultados com: Mostra resultados com: Índice

The Efficacy of Humanized Antibody against the Sporothrix Antigen, gp70, in Promoting Phagocytosis and Reducing Disease Burden

de Almeida, José R F ; Santiago, Karla L ; Kaihami, Gilberto H ; Maranhão, Andrea Q ; de Macedo Brígido, Marcelo ; de Almeida, Sandro R

Frontiers in microbiology, 2017-03, Vol.8, p.345-345 [Periódico revisado por pares]

Switzerland: Frontiers Media S.A

Texto completo disponível

Citações Citado por
  • Título:
    The Efficacy of Humanized Antibody against the Sporothrix Antigen, gp70, in Promoting Phagocytosis and Reducing Disease Burden
  • Autor: de Almeida, José R F ; Santiago, Karla L ; Kaihami, Gilberto H ; Maranhão, Andrea Q ; de Macedo Brígido, Marcelo ; de Almeida, Sandro R
  • Assuntos: Microbiology
  • É parte de: Frontiers in microbiology, 2017-03, Vol.8, p.345-345
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
    Edited by: Joshua D. Nosanchuk, Albert Einstein College of Medicine, USA
    This article was submitted to Fungi and Their Interactions, a section of the journal Frontiers in Microbiology
    Reviewed by: Joseph M. Bliss, Women & Infants Hospital of Rhode Island, USA; Marcos Sergio Toledo, Federal University of São Paulo, Brazil
    These authors have contributed equally to this work.
  • Descrição: Sporotrichosis is a subcutaneous mycosis distributed worldwide and is frequently reported in countries with tropical climates, as Latin America countries. We previously demonstrated that mice with sporotrichosis produce specific antibodies against a 70-kDa fungal protein, indicating that specific antibodies against this molecule may help to control the sporotrichosis. IgG1 monoclonal antibody was generated, and called mAbP6E7, in mice against a 70-kDa glycoprotein (gp70) of . The mAbP6E7 showed prophylactic and therapeutic activity against sporotrichosis. However, this antibody has a murine origin, and this can generate an immune response when administered to humans, precluding its use for a prolonged time. For its possible use in the treatment of human sporotrichosis, we humanized the mAbP6E7 by genetic engineering. Once expressed, the humanized antibodies had good stability and were able to bind to the 70-kDa cell wall antigens of and . The humanized P6E7 were able to opsonize yeasts, thus increasing the phagocytic index in human monocyte-derived macrophages. The treatment with humanized P6E7 decreased fungal burden . These data suggest that humanized P6E7 may have a therapeutic role in sporotrichosis.
  • Editor: Switzerland: Frontiers Media S.A
  • Idioma: Inglês
Links
Voltar para lista de resultados

  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

Buscando em bases de dados remotas. Favor aguardar.