Idioma:

A new Luminex‐based peptide assay to identify reactivity to baked, fermented, and whole milk

Sackesen, Cansin ; Suárez‐Fariñas, Mayte ; Silva, Ronaldo ; Lin, Jing ; Schmidt, Stephanie ; Getts, Robert ; Gimenez, Gustavo ; Yilmaz, Ebru A. ; Cavkaytar, Ozlem ; Buyuktiryaki, Betul ; Soyer, Ozge ; Grishina, Galina ; Sampson, Hugh A.

Allergy (Copenhagen), 2019-02, Vol.74 (2), p.327-336 [Periódico revisado por pares]

Denmark: Blackwell Publishing Ltd

Texto completo disponível

Citações Citado por

Enviar para

Título:
A new Luminex‐based peptide assay to identify reactivity to baked, fermented, and whole milk
Autor: Sackesen, Cansin ; Suárez‐Fariñas, Mayte ; Silva, Ronaldo ; Lin, Jing ; Schmidt, Stephanie ; Getts, Robert ; Gimenez, Gustavo ; Yilmaz, Ebru A. ; Cavkaytar, Ozlem ; Buyuktiryaki, Betul ; Soyer, Ozge ; Grishina, Galina ; Sampson, Hugh A.
Assuntos: Allergens - immunology ; Animals ; Assaying ; baked milk ; Cheese ; Children ; Cow's milk ; Cultured Milk Products - adverse effects ; Dairy products ; epitope ; Epitopes ; Female ; fermented milk ; Fermented milk products ; Humans ; Hypersensitivity ; Identification methods ; IgE ; IgG4 ; Immunoassay - methods ; Immunoglobulin E ; Immunoglobulin E - blood ; Immunoglobulin E - immunology ; Immunoglobulin G ; Immunoglobulin G - immunology ; Learning algorithms ; Luminescent Measurements - methods ; Male ; Milk ; Milk - adverse effects ; Milk Hypersensitivity - diagnosis ; Milk Hypersensitivity - immunology ; Milk products ; Model accuracy ; Peptides ; Phenotypes ; Protein Binding ; Proteins ; Reactivity ; Sensitivity and Specificity ; Yogurt
É parte de: Allergy (Copenhagen), 2019-02, Vol.74 (2), p.327-336
Notas: Funding information
Supported in part by the David H. and Julia Koch Research Program in Food Allergy Therapeutics.
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Background The majority of children with cow's milk allergy (CMA) tolerate baked milk. However, reactivity to fermented milk products such as yogurt/cheese has not been previously evaluated. We sought to determine whether children with CMA could tolerate yogurt/cheese and whether a patient's IgE and IgG4‐binding pattern to milk protein epitopes could distinguish clinical reactivity. Methods Four groups of reactivity were identified by Oral food challenge: baked milk reactive, fermented milk reactive, whole milk reactive, and outgrown. sIgE and sIgG4 binding to milk protein epitopes were assessed with a novel Luminex‐based peptide assay (LPA). Using machine learning techniques, a model was developed to predict different degrees of CMA. Results The baked milk reactive patients demonstrated the highest degree of IgE epitope binding, which was followed sequentially by fermented milk reactive, whole milk reactive, and outgrown. Data were randomly divided into two groups with 75% of the data utilized for model development (n = 68) and 25% for testing (n = 21). All 68 children used for training were correctly classified with models using IgE and IgG4 epitopes. The average cross‐validation accuracy was much higher for models using IgE plus IgG4 epitopes by LPA (84.8%), twice the performance of the serum component proteins assayed by UniCAP (41.9%). The performance of the model on “unseen data” was tested using the 21 withheld patients, and the accuracy of IgE was 86% (AUC = 0.89) while of IgE+IgG4 model was 81% (AUC = 0.94). Conclusion Using a novel high‐throughput LPA, we were able to distinguish the diversity of IgE/IgG4 binding to epitopes in the varying CMA phenotypes. LPA is a promising tool to predict correctly different degrees of CMA. The new food challenge sequence and Luminex‐based peptide assay to identify the clinical phenotypes of reactivity to baked, fermented, and whole cow's milk.
Editor: Denmark: Blackwell Publishing Ltd
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

A new Luminex‐based peptide assay to identify reactivity to baked, fermented, and whole milk

Sackesen, Cansin ; Suárez‐Fariñas, Mayte ; Silva, Ronaldo ; Lin, Jing ; Schmidt, Stephanie ; Getts, Robert ; Gimenez, Gustavo ; Yilmaz, Ebru A. ; Cavkaytar, Ozlem ; Buyuktiryaki, Betul ; Soyer, Ozge ; Grishina, Galina ; Sampson, Hugh A.

Denmark: Blackwell Publishing Ltd

Buscando em bases de dados remotas. Favor aguardar.