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NMR of All-Carbon-13 Sugars: An Application in Development of an Analytical Method for a Novel Natural Sugar, 1,5-Anhydrofructose

Kametani, Shunichi ; Mizuno, Hideaki ; Shiga, Yoko ; Akanuma, Hiroshi

Journal of biochemistry (Tokyo), 1996-01, Vol.119 (1), p.180-185 [Periódico revisado por pares]

England: Oxford University Press

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Título:
NMR of All-Carbon-13 Sugars: An Application in Development of an Analytical Method for a Novel Natural Sugar, 1,5-Anhydrofructose
Autor: Kametani, Shunichi ; Mizuno, Hideaki ; Shiga, Yoko ; Akanuma, Hiroshi
Assuntos: 13C-NMB ; 5-anhydro-D-fructose ; 5-anhydro-D-glucitol ; Animals ; Carbon Isotopes ; Deoxyglucose - analysis ; Deoxyglucose - chemistry ; Deoxyglucose - metabolism ; Fructose - analogs & derivatives ; Fructose - analysis ; Fructose - chemistry ; Fructose - metabolism ; GC-MS ; Liver - chemistry ; Magnetic Resonance Spectroscopy ; Mass Spectrometry ; Oxidation-Reduction ; polyol ; Rats
É parte de: Journal of biochemistry (Tokyo), 1996-01, Vol.119 (1), p.180-185
Notas: ark:/67375/HXZ-MSVW2K1S-4
istex:5805B572FCE7CEDD050EFABCFF2D0CF0D9A4400E
ArticleID:119.1.180
1Present address: Molecular Neurobiology Laboratory, Tsukuba Life Science Center, The Institute of Physical and Chemical Research (RIKEN), Tsukuba Life Science Center, 3-1-1 Koyadai, Tsukuba, Ibaraki 305.
ObjectType-Article-1
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Descrição: Of the all-carbon-13 compounds, glucose is one of the most easily accessible, and therefore we applied 13C-NMR technique to the metabolic study of glucose-related compounds, 1,5-anhydro-D-glucitol and 1,5-anhydro-D-fructose (AF). Applying an INADEQUATE method to the substitutes of these novel sugars fully labeled with carbon-13, we could trace out the entire carbon skeleton with high sensitivity and confirm the chemical structures of these sugars. The method also provided a much easier way to optimize the enzymatic oxidation for AF preparation: we selectively and continuously monitored the quantities, as well as their structures in aqueous solution, of the substrate and products in a noninvasive manner. Similarly relying upon information from the 13C-NME, we developed a valuable derivatization method of AF for its GC-MS application, which was so sensitive that we were able to demonstrate the natural occurrence of AF in rat liver.
Editor: England: Oxford University Press
Idioma: Inglês

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NMR of All-Carbon-13 Sugars: An Application in Development of an Analytical Method for a Novel Natural Sugar, 1,5-Anhydrofructose

Kametani, Shunichi ; Mizuno, Hideaki ; Shiga, Yoko ; Akanuma, Hiroshi

England: Oxford University Press

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