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BRASSINOSTEROID-SIGNALING KINASE 3, a plasma membrane-associated scaffold protein involved in early brassinosteroid signaling

Ren, Hong ; Willige, Björn C ; Jaillais, Yvon ; Geng, Sa ; Park, Mee Yeon ; Gray, William M ; Chory, Joanne Copenhaver, Gregory P.

PLoS genetics, 2019-01, Vol.15 (1), p.e1007904-e1007904 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
BRASSINOSTEROID-SIGNALING KINASE 3, a plasma membrane-associated scaffold protein involved in early brassinosteroid signaling
Autor: Ren, Hong ; Willige, Björn C ; Jaillais, Yvon ; Geng, Sa ; Park, Mee Yeon ; Gray, William M ; Chory, Joanne
Copenhaver, Gregory P.
Assuntos: Amino Acid Sequence - genetics ; Arabidopsis - genetics ; Arabidopsis - growth & development ; Arabidopsis Proteins - genetics ; Biology and Life Sciences ; Brassinosteroids ; Brassinosteroids - metabolism ; Cell division ; Cell membranes ; Cellular signal transduction ; Cloning ; Defects ; Fertility ; Gene expression ; Gene Expression Regulation, Plant ; Genetic research ; Genomics ; Hormones ; Kinases ; Laboratories ; Life Sciences ; Loss of Function Mutation - genetics ; Membrane proteins ; Mutation ; Myristoylation ; Observations ; Phenotype ; Phenotypes ; Phosphatase ; Phosphatases ; Phosphoprotein Phosphatases - genetics ; Phosphorylation ; Phosphotransferases ; Physiological aspects ; Physiology ; Plant biology ; Plants, Genetically Modified - genetics ; Plasma ; Protein-Serine-Threonine Kinases - genetics ; Proteins ; Research and Analysis Methods ; Signal Transduction ; Steroid hormones ; Steroids (Organic compounds) ; Transcription
É parte de: PLoS genetics, 2019-01, Vol.15 (1), p.e1007904-e1007904
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The authors have declared that no competing interests exist.
Descrição: Brassinosteroids (BRs) are steroid hormones essential for plant growth and development. The BR signaling pathway has been studied in some detail, however, the functions of the BRASSINOSTEROID-SIGNALING KINASE (BSK) family proteins in the pathway have remained elusive. Through forward genetics, we identified five semi-dominant mutations in the BSK3 gene causing BSK3 loss-of-function and decreased BR responses. We therefore investigated the function of BSK3, a receptor-like cytoplasmic kinase, in BR signaling and plant growth and development. We find that BSK3 is anchored to the plasma membrane via N-myristoylation, which is required for its function in BR signaling. The N-terminal kinase domain is crucial for BSK3 function, and the C-terminal three tandem TPR motifs contribute to BSK3/BSK3 homodimer and BSK3/BSK1 heterodimer formation. Interestingly, the effects of BSK3 on BR responses are dose-dependent, depending on its protein levels. Our genetic studies indicate that kinase dead BSK3K86R protein partially rescues the bsk3-1 mutant phenotypes. BSK3 directly interacts with the BSK family proteins (BSK3 and BSK1), BRI1 receptor kinase, BSU1 phosphatase, and BIN2 kinase. BIN2 phosphorylation of BSK3 enhances BSK3/BSK3 homodimer and BSK3/BSK1 heterodimer formation, BSK3/BRI1 interaction, and BSK3/BSU1 interaction. Furthermore, we find that BSK3 upregulates BSU1 transcript and protein levels to activate BR signaling. BSK3 is broadly expressed and plays an important role in BR-mediated root growth, shoot growth, and organ separation. Together, our findings suggest that BSK3 may function as a scaffold protein to regulate BR signaling. The results of our studies provide new insights into early BR signaling mechanisms.
Editor: United States: Public Library of Science
Idioma: Inglês

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BRASSINOSTEROID-SIGNALING KINASE 3, a plasma membrane-associated scaffold protein involved in early brassinosteroid signaling

Ren, Hong ; Willige, Björn C ; Jaillais, Yvon ; Geng, Sa ; Park, Mee Yeon ; Gray, William M ; Chory, Joanne Copenhaver, Gregory P.

United States: Public Library of Science

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