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A recombinant antigen with potential for serodiagnosis of Echinococcus granulosus infection in dogs

Gasser, R.B. ; Lightowlers, M.W. ; Rickard, M.D.

International journal for parasitology, 1990-11, Vol.20 (7), p.943-950 [Periódico revisado por pares]

Oxford: Elsevier Ltd

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Título:
A recombinant antigen with potential for serodiagnosis of Echinococcus granulosus infection in dogs
Autor: Gasser, R.B. ; Lightowlers, M.W. ; Rickard, M.D.
Assuntos: Animals ; Antibodies, Helminth - analysis ; antigens ; Antigens, Helminth - immunology ; Biological and medical sciences ; DNA libraries ; dog ; Dog Diseases - diagnosis ; Dogs ; Echinococcosis - diagnosis ; Echinococcosis - veterinary ; Echinococcus - immunology ; Echinococcus granulosus ; ELISA ; Enzyme-Linked Immunosorbent Assay ; glutathione transferase ; Helminthic diseases ; Infectious diseases ; Medical sciences ; Miscellaneous ; Parasitic diseases ; protoscoleces ; recombinant antigens ; recombinant DNA ; Recombinant Proteins - immunology ; serodiagnosis
É parte de: International journal for parasitology, 1990-11, Vol.20 (7), p.943-950
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Antibodies specific for Echinococcus granulosus were affinity purified from dog serum on transfer blots containing putative serodiagnostic antigens. These antibodies and serum pools derived from dogs with E. granulosus infections were used to screen a γt11 cDNA library constructed using E. granulosus protoscolex mRNA. Nine definitive antigenic clones were isolated and characterized, of which one (c10P1) gave strong specific reactions in plaque immunoassay with sera from E. granulosus infected dogs. These clones were all subcloned into the plasmid vector pGEX-1. Antigenicity of clones was confirmed in colony immunoassay and/or immunoblot. Glutathione S-transferase (GST) fusion proteins of individual subclones were produced in Escherichia coli, purified by affinity chromatography and evaluated in ELISA using sera from dogs with infections of E. granulosus, Taenia spp. or nematodes, and helminth-free dog sera. The GST fusion protein 10P1 showed a specificity of 100% in ELISA for diagnosis of E. granulosus infection in dogs despite its relatively low sensitivity. Further investigations aim to identify additional recombinant antigens and test 10P1 expressed in alternative expression systems to increase diagnostic sensitivity of the ELISA.
Editor: Oxford: Elsevier Ltd
Idioma: Inglês

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A recombinant antigen with potential for serodiagnosis of Echinococcus granulosus infection in dogs

Gasser, R.B. ; Lightowlers, M.W. ; Rickard, M.D.

Oxford: Elsevier Ltd

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