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An endosymbiont positively modulates ornithine decarboxylase in host trypanosomatids

Frossard, Mariana Lins ; Seabra, Sergio Henrique ; DaMatta, Renato Augusto ; de Souza, Wanderley ; de Mello, Fernando Garcia ; Motta, Maria Cristina Machado

Biochemical and biophysical research communications, 2006-05, Vol.343 (2), p.443-449 [Periódico revisado por pares]

United States: Elsevier Inc

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Título:
An endosymbiont positively modulates ornithine decarboxylase in host trypanosomatids
Autor: Frossard, Mariana Lins ; Seabra, Sergio Henrique ; DaMatta, Renato Augusto ; de Souza, Wanderley ; de Mello, Fernando Garcia ; Motta, Maria Cristina Machado
Assuntos: 60 APPLIED LIFE SCIENCES ; Animals ; ANTIBIOTICS ; BACTERIA ; Bacterial Physiological Phenomena ; Bacterial Proteins - metabolism ; BIOSYNTHESIS ; Cell growth ; CELL PROLIFERATION ; Crithidia - classification ; Crithidia - enzymology ; Crithidia - microbiology ; Crithidia deanei ; DECARBOXYLASES ; Endosymbiotic bacterium ; Enzyme Activation ; ENZYME ACTIVITY ; METABOLISM ; ORNITHINE ; Ornithine decarboxylase ; Ornithine Decarboxylase - metabolism ; Polyamine metabolism ; PROTOZOA ; Species Specificity ; Symbiosis - physiology ; Symbiotic relationship ; Trypanosomatid protozoa ; TRYPSIN
É parte de: Biochemical and biophysical research communications, 2006-05, Vol.343 (2), p.443-449
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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Descrição: Some trypanosomatids, such as Crithidia deanei, are endosymbiont-containing species. Aposymbiotic strains are obtained after antibiotic treatment, revealing interesting aspects of this symbiotic association. Ornithine decarboxylase (ODC) promotes polyamine biosynthesis and contributes to cell proliferation. Here, we show that ODC activity is higher in endosymbiont-bearing trypanosomatids than in aposymbiotic cells, but isolated endosymbionts did not display this enzyme activity. Intriguingly, expressed levels of ODC were similar in both strains, suggesting that ODC is positively modulated in endosymbiont-bearing cells. When the aposymbiotic strain was grown in conditioned medium, obtained after cultivation of the endosymbiont-bearing strain, cellular proliferation as well as ODC activity and localization were similar to that observed in the endosymbiont-containing trypanosomatids. Furthermore, dialyzed-heated medium and trypsin treatment reduced ODC activity of the aposymbiont strain. Taken together, these data indicate that the endosymbiont can enhance the protozoan ODC activity by providing factors of protein nature, which increase the host polyamine metabolism.
Editor: United States: Elsevier Inc
Idioma: Inglês

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An endosymbiont positively modulates ornithine decarboxylase in host trypanosomatids

Frossard, Mariana Lins ; Seabra, Sergio Henrique ; DaMatta, Renato Augusto ; de Souza, Wanderley ; de Mello, Fernando Garcia ; Motta, Maria Cristina Machado

United States: Elsevier Inc

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