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Comparison of Intra-CL Injection and Peripheral Application of Prostaglandin F2α Analog on Luteal Blood Flow and Secretory Function of the Bovine Corpus Luteum

Jonczyk, Agnieszka W. ; Piotrowska-Tomala, Katarzyna K. ; Skarzynski, Dariusz J.

Frontiers in veterinary science, 2022-01, Vol.8, p.811809-811809 [Periódico revisado por pares]

Frontiers Media S.A

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  • Título:
    Comparison of Intra-CL Injection and Peripheral Application of Prostaglandin F2α Analog on Luteal Blood Flow and Secretory Function of the Bovine Corpus Luteum
  • Autor: Jonczyk, Agnieszka W. ; Piotrowska-Tomala, Katarzyna K. ; Skarzynski, Dariusz J.
  • Assuntos: blood flow ; bovine ; corpus luteum ; oxytocin ; progesterone ; prostaglandin F2α ; Veterinary Science
  • É parte de: Frontiers in veterinary science, 2022-01, Vol.8, p.811809-811809
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
    Reviewed by: Mauricio Silva, Temuco Catholic University, Chile; Margherita Maranesi, University of Perugia, Italy
    Dariusz J. Skarzynski orcid.org/0000-0001-9537-3560
    Edited by: Arumugam Kumaresan, National Dairy Research Institute (ICAR), India
    ORCID: Agnieszka W. Jonczyk orcid.org/0000-0002-6867-0823
    This article was submitted to Animal Reproduction - Theriogenology, a section of the journal Frontiers in Veterinary Science
    Katarzyna K. Piotrowska-Tomala orcid.org/0000-0001-6989-9243
  • Descrição: We investigated the effects of different doses of dinoprost injected directly into the bovine corpus luteum (CL) on (i) concentrations of progesterone (P 4 ) and oxytocin (OT) in peripheral blood and (ii) mRNA levels of steroidogenic acute regulatory protein (STAR), cytochrome P450 family 11 subfamily A member 1 (P450scc), hydroxy-delta-5-steroid dehydrogenase, 3 β- and steroid delta-isomerase 1 (HSD3B), and receptor-interacting protein kinases 1 and 3 (RIPK1, RIPK3) in CL tissue. Moreover, we examined the effects of dinoprost, injected intra-CL or administered intramuscularly (IM), on CL secretory function and on indicators of CL vascular network status: luteal tissue area (LTA), CL blood flow (CLBF), and the CLBF:LTA ratio (Adj. CLBF), in cows at the early and mid-luteal phases. In the Experiment 1, cows (day 10 of the cycle) were allocated to (i) an intra-CL injection of saline (control; n = 3); (ii) an intra-CL injection of dinoprost (1.25 mg; 2.5 mg, or 5 mg; n = 3 for each dose); (iii) an IM administration of saline (control; n = 3); or (iv) an IM administration of dinoprost (25 mg; positive control; n = 3). Concentrations of OT and P 4 were measured in plasma samples. The mRNA expression of steroidogenesis- or necroptosis-related factors was determined in CL tissue 4 h after treatments. In Experiment 2, cows on day 4 ( n = 12) or day 10 ( n = 12) were allocated to (i) an intra-CL injection of dinoprost (2.5 mg/0.5 ml; n = 6), or (ii) IM administration of dinoprost (25 mg/5 ml; n = 6). Concentrations of P 4 were measured in plasma samples. Luteal tissue area, CLBF, and Adj. CLBF were assessed based on color Doppler ultrasonography. An intra-CL injection of dinoprost increased OT and decreased P 4 levels in the jugular vein (JV) in a dose-dependent manner in cows at the mid-luteal phase. Increased CLBF and Adj. CLBF, accompanied by reduced P 4 levels, were observed 2 h after intra-CL dinoprost injection in middle-stage CL. Decreased STAR and increased RIPK1 and RIPK3 mRNA levels confirmed that 2.5 mg dinoprost injected directly into CL is the minimum dose that induces luteolytic cascade. Injection of dinoprost directly into the CL (at a dosage lower than recommended for peripheral application) results in a pattern similar to IM dinoprost administration.
  • Editor: Frontiers Media S.A
  • Idioma: Inglês
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