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Enzymatic Determination of Anticholinesterases Using a Composite Carrier

Pitschmann, Vladimír ; Matějovský, Lukáš ; Vetchý, David ; Kobliha, Zbyněk

Analytical letters, 2016-10, Vol.49 (15), p.2418-2426 [Periódico revisado por pares]

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Título:
Enzymatic Determination of Anticholinesterases Using a Composite Carrier
Autor: Pitschmann, Vladimír ; Matějovský, Lukáš ; Vetchý, David ; Kobliha, Zbyněk
Assuntos: Carriers ; Cholinesterase ; Cholinesterase reaction ; composite carrier ; Eyes ; Indicators ; Mathematical analysis ; Physostigmine ; redox indicator ; Sarin ; Tubes
É parte de: Analytical letters, 2016-10, Vol.49 (15), p.2418-2426
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: A modified enzyme method is reported for the determination of cholinesterase inhibitors based on the use of a granulated composite carrier with immobilized butyrylcholinesterase. The detection system includes butyrylthiocholine as the substrate and N-(2,3-dimethyl-5-oxo-1-phenyl-3-pyrazoline-4-yl)-2-chloro-5-sulpho-4-iminobenzoquinone as a redox indicator. This method was characterized using carbamate physostigmine and sarin (O-isopropyl methylphosphonofluoridate). The method was also used in a simple tube detector. Evaluation was based on the observation with the naked eye of the decolorization of the redox indicator in comparison to a control time and by tristimulus colorimetry. The limits of detection were 0.5 µg/mL for physostigmine and 7 × 10 −4 mg/m 3 for sarin visually, or 0.06 µg/mL for physostigmine and 1.1 × 10 −4 mg/m 3 for sarin by colorimetry.
Editor: Taylor & Francis
Idioma: Inglês

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