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Direct visualization by cryo-EM of the mycobacterial capsular layer: a labile structure containing ESX-1-secreted proteins

Sani, Musa ; Houben, Edith N G ; Geurtsen, Jeroen ; Pierson, Jason ; de Punder, Karin ; van Zon, Maaike ; Wever, Brigitte ; Piersma, Sander R ; Jiménez, Connie R ; Daffé, Mamadou ; Appelmelk, Ben J ; Bitter, Wilbert ; van der Wel, Nicole ; Peters, Peter J Ramakrishnan, Lalita

PLoS pathogens, 2010-03, Vol.6 (3), p.e1000794-e1000794 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Direct visualization by cryo-EM of the mycobacterial capsular layer: a labile structure containing ESX-1-secreted proteins
Autor: Sani, Musa ; Houben, Edith N G ; Geurtsen, Jeroen ; Pierson, Jason ; de Punder, Karin ; van Zon, Maaike ; Wever, Brigitte ; Piersma, Sander R ; Jiménez, Connie R ; Daffé, Mamadou ; Appelmelk, Ben J ; Bitter, Wilbert ; van der Wel, Nicole ; Peters, Peter J
Ramakrishnan, Lalita
Assuntos: Bacteria ; Bacterial Capsules - metabolism ; Bacterial Capsules - ultrastructure ; Bacterial Outer Membrane Proteins - metabolism ; Bacterial Outer Membrane Proteins - secretion ; Bacterial Outer Membrane Proteins - ultrastructure ; Bacterial proteins ; Bacteriology ; Cell Biology ; Cryoelectron Microscopy ; Cytokines ; Cytokines - metabolism ; Electron microscopy ; Experiments ; Health aspects ; Humans ; Infectious Diseases/Bacterial Infections ; Lipids ; Macrophages - immunology ; Macrophages - metabolism ; Macrophages - microbiology ; Microbiology ; Microbiology/Cellular Microbiology and Pathogenesis ; Microscopy ; Microscopy, Immunoelectron ; Mycobacteria ; Mycobacterium ; Mycobacterium - metabolism ; Mycobacterium - ultrastructure ; Mycobacterium bovis - metabolism ; Mycobacterium bovis - ultrastructure ; Mycobacterium marinum - metabolism ; Mycobacterium marinum - ultrastructure ; Mycobacterium smegmatis - metabolism ; Mycobacterium smegmatis - ultrastructure ; Mycobacterium tuberculosis - metabolism ; Mycobacterium tuberculosis - ultrastructure ; Physiological aspects ; Proteins ; Structure ; Vaccines
É parte de: PLoS pathogens, 2010-03, Vol.6 (3), p.e1000794-e1000794
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Conceived and designed the experiments: MS ENGH JG MD BJA WB NvdW PJP. Performed the experiments: MS ENGH JG JP KdP MvZ BW SRP CRJ. Analyzed the data: MS ENGH JG KdP NvdW. Wrote the paper: MS ENGH JG SRP MD BJA WB NvdW PJP.
Current address: Université de Toulouse (Toulouse III), Toulouse, France
Descrição: The cell envelope of mycobacteria, a group of Gram positive bacteria, is composed of a plasma membrane and a Gram-negative-like outer membrane containing mycolic acids. In addition, the surface of the mycobacteria is coated with an ill-characterized layer of extractable, non-covalently linked glycans, lipids and proteins, collectively known as the capsule, whose occurrence is a matter of debate. By using plunge freezing cryo-electron microscopy technique, we were able to show that pathogenic mycobacteria produce a thick capsule, only present when the cells were grown under unperturbed conditions and easily removed by mild detergents. This detergent-labile capsule layer contains arabinomannan, alpha-glucan and oligomannosyl-capped glycolipids. Further immunogenic and proteomic analyses revealed that Mycobacterium marinum capsule contains high amounts of proteins that are secreted via the ESX-1 pathway. Finally, cell infection experiments demonstrated the importance of the capsule for binding to cells and dampening of pro-inflammatory cytokine response. Together, these results show a direct visualization of the mycobacterial capsular layer as a labile structure that contains ESX-1-secreted proteins.
Editor: United States: Public Library of Science
Idioma: Inglês

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Direct visualization by cryo-EM of the mycobacterial capsular layer: a labile structure containing ESX-1-secreted proteins

Sani, Musa ; Houben, Edith N G ; Geurtsen, Jeroen ; Pierson, Jason ; de Punder, Karin ; van Zon, Maaike ; Wever, Brigitte ; Piersma, Sander R ; Jiménez, Connie R ; Daffé, Mamadou ; Appelmelk, Ben J ; Bitter, Wilbert ; van der Wel, Nicole ; Peters, Peter J Ramakrishnan, Lalita

United States: Public Library of Science

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