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Single muscle fiber proteomics reveals unexpected mitochondrial specialization

Murgia, Marta ; Nagaraj, Nagarjuna ; Deshmukh, Atul S ; Zeiler, Marlis ; Cancellara, Pasqua ; Moretti, Irene ; Reggiani, Carlo ; Schiaffino, Stefano ; Mann, Matthias

EMBO reports, 2015-03, Vol.16 (3), p.387-395 [Periódico revisado por pares]

England: Blackwell Publishing Ltd

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  • Título:
    Single muscle fiber proteomics reveals unexpected mitochondrial specialization
  • Autor: Murgia, Marta ; Nagaraj, Nagarjuna ; Deshmukh, Atul S ; Zeiler, Marlis ; Cancellara, Pasqua ; Moretti, Irene ; Reggiani, Carlo ; Schiaffino, Stefano ; Mann, Matthias
  • Assuntos: Animals ; Chromatography, Liquid ; Computational Biology - methods ; exercise ; Immunohistochemistry ; Mass Spectrometry ; Metabolism ; Mice ; Mitochondria ; Mitochondria - metabolism ; muscle fibers ; Muscle Fibers, Skeletal - metabolism ; Proteome - genetics ; Proteome - metabolism ; Proteomics ; Proteomics - methods ; Scientific Reports ; single cell
  • É parte de: EMBO reports, 2015-03, Vol.16 (3), p.387-395
  • Notas: ArticleID:EMBR201439757
    Supplementary InformationSupplementary Table S1Supplementary Table S2Supplementary Table S3Supplementary Table S4Source data for Supplementary Figure S3BReview Process FileSource data for Figure 3
    ark:/67375/WNG-TN62N7C7-V
    EC 7th Framework Programme - No. HEALTH-F4-2008-201648/PROSPECTS
    Novo Nordisk Foundation Center for Basic Metabolic Research, Copenhagen, Denmark
    The Louis Jantet Foundation
    Max-Planck Society for the Advancement of Science
    istex:E32C252B9C23FB3F801AEE87C62577B9E37C5A99
    ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
    Subject Categories Post-translational Modifications, Proteolysis & Proteomics
  • Descrição: Mammalian skeletal muscles are composed of multinucleated cells termed slow or fast fibers according to their contractile and metabolic properties. Here, we developed a high‐sensitivity workflow to characterize the proteome of single fibers. Analysis of segments of the same fiber by traditional and unbiased proteomics methods yielded the same subtype assignment. We discovered novel subtype‐specific features, most prominently mitochondrial specialization of fiber types in substrate utilization. The fiber type‐resolved proteomes can be applied to a variety of physiological and pathological conditions and illustrate the utility of single cell type analysis for dissecting proteomic heterogeneity. Synopsis Here, single multinucleated muscle fibers are analyzed by high sensitivity proteomics for the first time. This reveals an unexpected diversity and specialization in metabolic properties of individual fiber types, in particular in mitochondrial function. First single skeletal muscle fiber proteome, and thereby single cell proteome of any kind Single‐shot proteomic analysis captures main features of type 1‐slow and of the subtypes of fast fibers Qualitative differences in the mitochondrial proteome of the four fiber types uncover a metabolic specialization Here, single multinucleated muscle fibers are analyzed by high sensitivity proteomics for the first time. This reveals an unexpected diversity and specialization in metabolic properties of individual fiber types, in particular in mitochondrial function.
  • Editor: England: Blackwell Publishing Ltd
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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