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Release of Escherichia coli DNA from Membrane Complexes by Single-Strand Endonucleases

Abe, M. ; Brown, C. ; Hendrickson, W. G. ; Boyd, D. H. ; Clifford, P. ; Cote, R. H. ; Schaechter, M.

Proceedings of the National Academy of Sciences - PNAS, 1977-07, Vol.74 (7), p.2756-2760 [Periódico revisado por pares]

United States: National Academy of Sciences of the United States of America

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Título:
Release of Escherichia coli DNA from Membrane Complexes by Single-Strand Endonucleases
Autor: Abe, M. ; Brown, C. ; Hendrickson, W. G. ; Boyd, D. H. ; Clifford, P. ; Cote, R. H. ; Schaechter, M.
Assuntos: Acetates ; Animals ; Aspergillus oryzae - enzymology ; Cell Membrane - metabolism ; Chromosome Mapping ; Chromosomes ; Crystals ; DNA ; DNA Replication ; DNA, Bacterial - metabolism ; DNA, Single-Stranded - metabolism ; Endonucleases - metabolism ; Enzymes ; Escherichia coli - metabolism ; Escherichia coli - ultrastructure ; Fishes ; Fractions ; Liver - enzymology ; Molecular Weight ; Nuclear membrane ; P branes ; Plants - enzymology ; Replication origin
É parte de: Proceedings of the National Academy of Sciences - PNAS, 1977-07, Vol.74 (7), p.2756-2760
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Treatment of gently prepared lysates of Escherichia coli with single-strand-specific endonuclease (S1 or from mung beans) results in the release of about 90% of the DNA from membranes, as determined by the M band technique. The released DNA has an average molecular weight of about 1.2 × 108. Data obtained with endonuclease S1 fit a mathematical model in which substrate sites are at or near membrane attachment sites. Data obtained with pancreatic deoxyribonuclease or x-rays fit a model for double-strand breaks at random sites along the DNA. Fitting data to these models, we estimate that there are 18 ± 5 membrane attachment sites. The DNA remaining after S1 nuclease treatment is enriched for the region near the origin of chromosome replication. Therefore, attachment at this region appears to be chemically different from that at the other sites along the DNA.
Editor: United States: National Academy of Sciences of the United States of America
Idioma: Inglês

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Release of Escherichia coli DNA from Membrane Complexes by Single-Strand Endonucleases

Abe, M. ; Brown, C. ; Hendrickson, W. G. ; Boyd, D. H. ; Clifford, P. ; Cote, R. H. ; Schaechter, M.

United States: National Academy of Sciences of the United States of America

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