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Proteomics reveals the effects of gibberellic acid (GA 3) on salt-stressed rice ( Oryza sativa L.) shoots

Wen, Fu-ping ; Zhang, Zhao-hui ; Bai, Ting ; Xu, Qin ; Pan, Ying-hong

Plant science (Limerick), 2010-02, Vol.178 (2), p.170-175 [Periódico revisado por pares]

Shannon: Elsevier Ireland Ltd

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Título:
Proteomics reveals the effects of gibberellic acid (GA 3) on salt-stressed rice ( Oryza sativa L.) shoots
Autor: Wen, Fu-ping ; Zhang, Zhao-hui ; Bai, Ting ; Xu, Qin ; Pan, Ying-hong
Assuntos: application rate ; biochemical pathways ; Biological and medical sciences ; enzyme activity ; enzymes ; Fundamental and applied biological sciences. Psychology ; gene expression regulation ; gibberellic acid ; Gibberellic acid (GA3) ; grain crops ; mechanism of action ; Oryza sativa ; plant growth ; plant proteins ; protein synthesis ; proteome ; Proteomics ; rice ; Rice ( Oryza sativa L.) ; root growth ; Salt stress ; seedlings ; shoots ; sodium chloride
É parte de: Plant science (Limerick), 2010-02, Vol.178 (2), p.170-175
Notas: http://dx.doi.org/10.1016/j.plantsci.2009.11.006
Descrição: Salt decreases gibberellic acid (GA 3) content in rice. Nevertheless, GA 3 may exert a beneficial effect on salt-stressed rice. In our analysis, GA 3 reduced NaCl-induced growth inhibition in rice ( Oryza sativa L. cv. Nipponbare) in a concentration-dependent manner, including the length of root tissue. However, remains unclear. We employed a comparative proteomic analysis to elucidate the mechanism of GA 3 activity in this phenomenon. 5-Day-old seedlings treated with salt and gibberellic acid (H 2O, 0.5% NaCl, 0.5% NaCl + 100 μM GA 3, and 100 μM GA 3) for 48 h were used for the proteomics analysis. Eleven proteins differently regulated by salt and GA 3 were revealed by 2D PAGE and were identified by MALDI-TOF MS. These proteins were identified as glutamyl-tRNA reductase, enolase, salt stress-induced protein (SALT protein), Os09g0249700, hypothetical protein OsJ_014066, putative chaperonin 21 precursor, Os04g0659300, hypothetical protein OsJ_025258, ribulose bisphosphate carboxylase, isoflavone reductase-like protein and phosphoglucomutase. Some of these proteins are involved in biochemical pathways such as photosynthesis and glycolysis, but others were found to be novel proteins involved specifically in the response to salt in rice. GA 3 had a significant influence on the abundance of some salt-regulated proteins. Our studies have provided new insight to reveal the modulating effect of GA 3 on salt stress in rice.
Editor: Shannon: Elsevier Ireland Ltd
Idioma: Inglês

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Proteomics reveals the effects of gibberellic acid (GA 3) on salt-stressed rice ( Oryza sativa L.) shoots

Wen, Fu-ping ; Zhang, Zhao-hui ; Bai, Ting ; Xu, Qin ; Pan, Ying-hong

Shannon: Elsevier Ireland Ltd

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