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Engineering and application of a biosensor with focused ligand specificity

Della Corte, Dennis ; van Beek, Hugo L. ; Syberg, Falk ; Schallmey, Marcus ; Tobola, Felix ; Cormann, Kai U. ; Schlicker, Christine ; Baumann, Philipp T. ; Krumbach, Karin ; Sokolowsky, Sascha ; Morris, Connor J. ; Grünberger, Alexander ; Hofmann, Eckhard ; Schröder, Gunnar F. ; Marienhagen, Jan

Nature communications, 2020-09, Vol.11 (1), p.4851-4851, Article 4851 [Periódico revisado por pares]

London: Nature Publishing Group UK

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  • Título:
    Engineering and application of a biosensor with focused ligand specificity
  • Autor: Della Corte, Dennis ; van Beek, Hugo L. ; Syberg, Falk ; Schallmey, Marcus ; Tobola, Felix ; Cormann, Kai U. ; Schlicker, Christine ; Baumann, Philipp T. ; Krumbach, Karin ; Sokolowsky, Sascha ; Morris, Connor J. ; Grünberger, Alexander ; Hofmann, Eckhard ; Schröder, Gunnar F. ; Marienhagen, Jan
  • É parte de: Nature communications, 2020-09, Vol.11 (1), p.4851-4851, Article 4851
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
  • Descrição: Abstract Cell factories converting bio-based precursors to chemicals present an attractive avenue to a sustainable economy, yet screening of genetically diverse strain libraries to identify the best-performing whole-cell biocatalysts is a low-throughput endeavor. For this reason, transcriptional biosensors attract attention as they allow the screening of vast libraries when used in combination with fluorescence-activated cell sorting (FACS). However, broad ligand specificity of transcriptional regulators (TRs) often prohibits the development of such ultra-high-throughput screens. Here, we solve the structure of the TR LysG of Corynebacterium glutamicum , which detects all three basic amino acids. Based on this information, we follow a semi-rational engineering approach using a FACS-based screening/counterscreening strategy to generate an l -lysine insensitive LysG-based biosensor. This biosensor can be used to isolate l -histidine-producing strains by FACS, showing that TR engineering towards a more focused ligand spectrum can expand the scope of application of such metabolite sensors.
  • Editor: London: Nature Publishing Group UK
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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