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IS-pro: high-throughput molecular fingerprinting of the intestinal microbiota

Budding, A.E ; Grasman, M.E ; Lin, F ; Bogaards, J.A ; Soeltan-Kaersenhout, D.J ; Vandenbroucke-Grauls, C.M.J.E ; van Bodegraven, A.A ; Savelkoul, P.H.M

The FASEB journal, 2010-11, Vol.24 (11), p.4556-4564 [Periódico revisado por pares]

United States: The Federation of American Societies for Experimental Biology

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Título:
IS-pro: high-throughput molecular fingerprinting of the intestinal microbiota
Autor: Budding, A.E ; Grasman, M.E ; Lin, F ; Bogaards, J.A ; Soeltan-Kaersenhout, D.J ; Vandenbroucke-Grauls, C.M.J.E ; van Bodegraven, A.A ; Savelkoul, P.H.M
Assuntos: 16S–23S IS region ; Bacteria - classification ; Bacteria - genetics ; Biodiversity ; complex microbial communities ; DNA Fingerprinting - methods ; DNA, Ribosomal Spacer - genetics ; Electrophoresis, Capillary - methods ; Feces - microbiology ; gut bacteria ; Humans ; Intestines - microbiology ; phylum sorting ; Polymerase Chain Reaction - methods ; profiling ; Reproducibility of Results ; Species Specificity
É parte de: The FASEB journal, 2010-11, Vol.24 (11), p.4556-4564
Notas: ObjectType-Article-1
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Descrição: The human intestinal microbiota is known to play an important role in human health and disease, and with the advent of novel molecular techniques, disease-specific variations in its composition have been found. However, analysis of the intestinal microbiota has not yet been applicable in large-scale clinical research or routine diagnostics because of the complex and expensive nature of the techniques needed. Here, we describe a new PCR-based profiling technique for high-throughput analysis of the human intestinal microbiota, which we have termed IS-pro. This technique combines bacterial species differentiation by the length of the 16S-23S rDNA interspace region with instant taxonomic classification by phylum-specific fluorescent labeling of PCR primers. We validated IS-pro in silico, in vitro, and in vivo, on human colonic biopsies and feces, and introduced a standardized protocol for data analysis. IS-pro is easy to implement in general clinical microbiological laboratories with access to capillary gel electrophoresis, and the high-throughput nature of the test makes analysis of large numbers of samples feasible. This combination renders IS-pro ideally suited for use in clinical research and routine diagnostics.--Budding, A. E., Grasman, M. E., Lin, F., Bogaards, J. A., Soeltan-Kaersenhout, D. J., Vandenbroucke-Grauls, C. M. J. E., van Bodegraven, A. A., Savelkoul, P. H. M. IS-pro: high-throughput molecular fingerprinting of the intestinal microbiota.
Editor: United States: The Federation of American Societies for Experimental Biology
Idioma: Inglês

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IS-pro: high-throughput molecular fingerprinting of the intestinal microbiota

Budding, A.E ; Grasman, M.E ; Lin, F ; Bogaards, J.A ; Soeltan-Kaersenhout, D.J ; Vandenbroucke-Grauls, C.M.J.E ; van Bodegraven, A.A ; Savelkoul, P.H.M

United States: The Federation of American Societies for Experimental Biology

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