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Next generation sequencing for miRNA profile of spleen CD4 + T cells in the murine model of acute asthma

Liu, Ye ; Chen, Zhongqi ; Xu, Kun ; Wang, Zhengxia ; Wu, Chaojie ; Sun, Zhixiao ; Ji, Ningfei ; Huang, Mao ; Zhang, Mingshun

Epigenomics, 2018-08, Vol.10 (8), p.1071-1083 [Periódico revisado por pares]

England: Future Medicine Ltd

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Título:
Next generation sequencing for miRNA profile of spleen CD4 + T cells in the murine model of acute asthma
Autor: Liu, Ye ; Chen, Zhongqi ; Xu, Kun ; Wang, Zhengxia ; Wu, Chaojie ; Sun, Zhixiao ; Ji, Ningfei ; Huang, Mao ; Zhang, Mingshun
Assuntos: Acids ; Allergies ; Animal models ; Asthma ; Binding sites ; Bioinformatics ; CD4 antigen ; Gene expression ; Genomes ; Kinases ; Lymphocytes ; Lymphocytes T ; MicroRNAs ; miRNA ; Next-generation sequencing ; Ontology ; Polymerase chain reaction ; Reverse transcription ; Spleen ; Transfer RNA
É parte de: Epigenomics, 2018-08, Vol.10 (8), p.1071-1083
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: To explore the miRNAs profile of CD4 T lymphocytes in asthma via next generation sequencing. In the murine model of acute asthma, spleen CD4 T lymphocytes were sorted, in which small RNAs were extracted and sequenced. Novel miRNAs were measured with real time quantitative reverse transcription polymerase chain reaction (qRT-PCR). A total of 127 miRNAs were found to exhibit at least twofold change. In the 262 predicted novel miRNAs, 14 novel miRNAs were measured in qRT-PCR in the sorted CD4 T cells or in the differentiated Th1/Th2 cells and novel miR-11 (xxx-m0228-3p) was significantly decreased in the sorted CD4 T cells from the murine model of asthma and in the Th2 cells. Aberrant miRNAs profile in the CD4 T lymphocytes from acute asthma was documented.
Editor: England: Future Medicine Ltd
Idioma: Inglês

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Next generation sequencing for miRNA profile of spleen CD4 + T cells in the murine model of acute asthma

Liu, Ye ; Chen, Zhongqi ; Xu, Kun ; Wang, Zhengxia ; Wu, Chaojie ; Sun, Zhixiao ; Ji, Ningfei ; Huang, Mao ; Zhang, Mingshun

England: Future Medicine Ltd

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