skip to main content
Idioma:
Primo Search
Clear Search Box
Search in: Busca Geral
Busca Avançada
Busca por Índices

Deep phenotyping of two pre-clinical mouse models and a cohort of RBM20 mutation carriers reveals no sex-dependent disease severity in RBM20 cardiomyopathy

Lennermann, David C. ; Pepin, Mark E. ; Grosch, Markus ; Konrad, Laura ; Kemmling, Elena ; Hartmann, Joshua ; Nolte, Janica L. ; Clauder-Münster, Sandra ; Kayvanpour, Elham ; Sedaghat-Hamedani, Farbod ; Haas, Jan ; Meder, Benjamin ; van den Boogaard, Malou ; Amin, Ahmad S. ; Dewenter, Matthias ; Krüger, Marcus ; Steinmetz, Lars M. ; Backs, Johannes ; van den Hoogenhof, Maarten M.G.

American journal of physiology. Heart and circulatory physiology, 2022-11 [Periódico revisado por pares]

Texto completo disponível

Citações Citado por
  • Título:
    Deep phenotyping of two pre-clinical mouse models and a cohort of RBM20 mutation carriers reveals no sex-dependent disease severity in RBM20 cardiomyopathy
  • Autor: Lennermann, David C. ; Pepin, Mark E. ; Grosch, Markus ; Konrad, Laura ; Kemmling, Elena ; Hartmann, Joshua ; Nolte, Janica L. ; Clauder-Münster, Sandra ; Kayvanpour, Elham ; Sedaghat-Hamedani, Farbod ; Haas, Jan ; Meder, Benjamin ; van den Boogaard, Malou ; Amin, Ahmad S. ; Dewenter, Matthias ; Krüger, Marcus ; Steinmetz, Lars M. ; Backs, Johannes ; van den Hoogenhof, Maarten M.G.
  • É parte de: American journal of physiology. Heart and circulatory physiology, 2022-11
  • Descrição: Aims RBM20 cardiomyopathy is an arrhythmogenic form of dilated cardiomyopathy caused by mutations in the splicing factor RBM20. A recent study found a more severe phenotype in male RBM20 cardiomyopathy patients than in female patients. Here, we aim to determine sex differences in an animal model of RBM20 cardiomyopathy, and investigate potential underlying mechanisms. Additionally, we aim to determine sex and gender differences in clinical parameters in a novel RBM20 cardiomyopathy patient cohort. Methods and Results We characterized a Rbm20 knockout (KO) mouse model, and show that splicing of key RBM20 targets, cardiac function, and arrhythmia susceptibility do not differ between sexes. Next, we performed deep phenotyping of these mice, and show that male and female Rbm20-KO mice possess transcriptomic and phosphoproteomic differences. Hypothesizing that these differences may influence the heart's ability to compensate for stress, we exposed Rbm20-KO mice to acute catecholaminergic stimulation, and again found no functional differences. We also replicate the lack of functional differences in a mouse model with the Rbm20-R636Q mutation. Lastly, we present a patient cohort of 33 RBM20 cardiomyopathy patients, and show that these patients do not possess sex and gender differences in disease severity. Conclusions Current mouse models of RBM20 cardiomyopathy show more pronounced changes in gene expression and phosphorylation of cardiac proteins in male mice, but no sex differences in cardiac morphology and function. Moreover, other than reported before, male RBM20 cardiomyopathy patients do not present with worse cardiac function in a patient cohort from Germany and the Netherlands.
  • Idioma: Inglês
Voltar para lista de resultados

  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

Buscando em bases de dados remotas. Favor aguardar.