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Identification, Stereoconfiguration, Chromatographic and Fluorescence Properties of Natural Pterins

Klein, Roger

Advances in experimental medicine and biology, 1993, Vol.338, p.43-46 [Periódico revisado por pares]

Boston, MA: Springer US

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  • Título:
    Identification, Stereoconfiguration, Chromatographic and Fluorescence Properties of Natural Pterins
  • Autor: Klein, Roger
  • Assuntos: Chromatography, High Pressure Liquid ; Fluorescence Property ; Fluorescence Quantum Yield ; Fluorescent Peak ; Human Urine ; Pterins - chemistry ; Pterins - isolation & purification ; Quantum Theory ; Spectrometry, Fluorescence ; Stereoisomerism ; Structure-Activity Relationship ; Urine Sample
  • É parte de: Advances in experimental medicine and biology, 1993, Vol.338, p.43-46
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
  • Descrição: Several years ago, we reported the identification of dictyopterin as 6-[D-threo]-1’, 2’-dihydroxy propyl-pterin1. Such a result had been obtained after numerous and tedious purification steps. In order to facilitate the determination of the stereoconfiguration of natural pterins, which are found in very small amounts in living organisms, we aimed to find a sensitive method. This was achieved in using chiral HPLC. The separation of D-and L-enantiomers of 6-(polyhydroxypropyl)-pterins was obtained by ligand exchange chromatography in using a reversed-phase column with a mobile phase containing D-phenylalanine as the chiral modifier and Cu(II) as the metal ion. This enantiomeric separation allowed the stereoconfiguration of some natural pterins to be deterriiined in the picomole range, by comparison of their chromatographic and fluorescence properties with those of reference enantiomeric pterins2. The present paper briefly reports an aspect of the method dealing with the fluorescence quantum yields of pterins and describes one application which answers the following questions : what is the stereoconfiguration of urinary monapterin ? Are there any differences in human urine from cancer and non-cancer patients?
  • Editor: Boston, MA: Springer US
  • Idioma: Inglês
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