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A Two-Dimensional Metallacycle Cross-Linked Switchable Polymer for Fast and Highly Efficient Phosphorylated Peptide Enrichment

Chen, Li-Jun ; Humphrey, Sean J ; Zhu, Jun-Long ; Zhu, Fan-Fan ; Wang, Xu-Qing ; Wang, Xiang ; Wen, Jin ; Yang, Hai-Bo ; Gale, Philip A

Journal of the American Chemical Society, 2021-06, Vol.143 (22), p.8295-8304 [Periódico revisado por pares]

United States: American Chemical Society

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  • Título:
    A Two-Dimensional Metallacycle Cross-Linked Switchable Polymer for Fast and Highly Efficient Phosphorylated Peptide Enrichment
  • Autor: Chen, Li-Jun ; Humphrey, Sean J ; Zhu, Jun-Long ; Zhu, Fan-Fan ; Wang, Xu-Qing ; Wang, Xiang ; Wen, Jin ; Yang, Hai-Bo ; Gale, Philip A
  • Assuntos: Cross-Linking Reagents - chemistry ; Microscopy, Electron, Transmission ; Molecular Structure ; Organoplatinum Compounds - chemistry ; Phosphopeptides - chemical synthesis ; Phosphopeptides - chemistry ; Phosphorylation ; Polymers - chemistry
  • É parte de: Journal of the American Chemical Society, 2021-06, Vol.143 (22), p.8295-8304
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
  • Descrição: The selective and efficient capture of phosphopeptides is critical for comprehensive and in-depth phosphoproteome analysis. Here we report a new switchable two-dimensional (2D) supramolecular polymer that serves as an ideal platform for the enrichment of phosphopeptides. A well-defined, positively charged metallacycle incorporated into the polymer endows the resultant polymer with a high affinity for phosphopeptides. Importantly, the stimuli-responsive nature of the polymer facilitates switchable binding affinity of phosphopeptides, thus resulting in an excellent performance in phosphopeptide enrichment and separation from model proteins. The polymer has a high enrichment capacity (165 mg/g) and detection sensitivity (2 fmol), high enrichment recovery (88%), excellent specificity, and rapid enrichment and separation properties. Additionally, we have demonstrated the capture of phosphopeptides from the tryptic digest of real biosamples, thus illustrating the potential of this polymeric material in phosphoproteomic studies.
  • Editor: United States: American Chemical Society
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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