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17-β-Estradiol alters Jurkat lymphocyte cell cycling and induces apoptosis through suppression of Bcl-2 and cyclin A

Jenkins, John K ; Suwannaroj, Siraphop ; Elbourne, Keith B ; Ndebele, Kenneth ; McMurray, Robert W

International immunopharmacology, 2001-10, Vol.1 (11), p.1897-1911 [Periódico revisado por pares]

Amsterdam: Elsevier B.V

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Título:
17-β-Estradiol alters Jurkat lymphocyte cell cycling and induces apoptosis through suppression of Bcl-2 and cyclin A
Autor: Jenkins, John K ; Suwannaroj, Siraphop ; Elbourne, Keith B ; Ndebele, Kenneth ; McMurray, Robert W
Assuntos: Antineoplastic agents ; Apoptosis ; Apoptosis - drug effects ; Bcl-2 protein ; Biological and medical sciences ; Blotting, Western ; CD4 antigen ; Cell cycle ; Cell Cycle - drug effects ; cyclin A ; Cyclin A - antagonists & inhibitors ; Cyclin A - biosynthesis ; DNA Fragmentation ; Estradiol - pharmacology ; Estrogen ; General aspects ; Humans ; Jurkat Cells ; Medical sciences ; Pharmacology. Drug treatments ; Progesterone ; Progesterone - pharmacology ; propidium iodide ; Proto-Oncogene Proteins c-bcl-2 - antagonists & inhibitors ; Proto-Oncogene Proteins c-bcl-2 - biosynthesis ; RNA Probes ; T lymphocytes
É parte de: International immunopharmacology, 2001-10, Vol.1 (11), p.1897-1911
Notas: ObjectType-Article-2
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Descrição: In this investigation, the effects and potential mechanisms of female sex steroid action on proliferation, cell cycling, and apoptosis in Jurkat CD4+ T lymphocytes were examined. 17-β-Estradiol (estrogen) inhibited Jurkat T cell proliferation, stimulated accumulation of cells in S and G 2/M phases of the cell cycle, and induced apoptosis over 72 h in a dose-dependent manner. 4-Pregnene-3,20-dione (progesterone) did not induce redistribution of the cells in the cell cycle but did induce cytostasis and slightly increased apoptosis. Simultaneous staining with anti-BrDU and propidium iodide indicated that estrogen-treated Jurkat T cells proceeded through S phase prior to apoptosis. Progesterone halted cell cycle progression; cells did not progress through S phase or incorporate BrDU. Both hormones decreased the percentage of cells in S or G 2/M expressing cyclin A protein, but did not affect cyclin D protein expression. Cyclin A mRNA was markedly decreased by estrogen. Bcl-2 protein and mRNA were also reduced in estrogen but not progesterone-treated Jurkat T lymphocytes. This data shows that high concentrations of estrogen or progesterone significantly suppress lymphoproliferation in association with suppression of cyclin A. Additionally, bcl-2 protein levels were suppressed in association with estrogen-induced apoptosis. These findings demonstrate direct, hormone-specific effects on lymphocytes that may provide insight into their role in immunomodulation or the development of autoimmunity.
Editor: Amsterdam: Elsevier B.V
Idioma: Inglês

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17-β-Estradiol alters Jurkat lymphocyte cell cycling and induces apoptosis through suppression of Bcl-2 and cyclin A

Jenkins, John K ; Suwannaroj, Siraphop ; Elbourne, Keith B ; Ndebele, Kenneth ; McMurray, Robert W

Amsterdam: Elsevier B.V

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