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Promoter regions of plasmodium vivax are unable to efficiently recruit the transcriptional machinery of P. falciparum

Mauro Ferreira de Azevedo Hernando Antonio Del Portillo; Congresso do Instituto de Ciências Biomédicas (4. 2005 São Paulo)

Resumos São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2005

São Paulo Comissão de Cultura e Extensão Universitária do ICB/USP 2005

Item não circula. Consulte sua biblioteca.(Acessar)

  • Título:
    Promoter regions of plasmodium vivax are unable to efficiently recruit the transcriptional machinery of P. falciparum
  • Autor: Mauro Ferreira de Azevedo
  • Hernando Antonio Del Portillo; Congresso do Instituto de Ciências Biomédicas (4. 2005 São Paulo)
  • Assuntos: PARASITOLOGIA
  • É parte de: Resumos São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2005
  • Notas Locais: Disponível somente em CD-ROM
  • Descrição: Promoters of malaria parasites contain conserved regulatory elements as promoters from different Plasmodium species can drive expression of reporter genes in heterologous transfection systems. Here we describe transient transfections in P. falciparum with luciferase reporter plasmids containing intergenic regions of the msp1, dhfr, vir3, vir24 and ef1-alpha genes of P. vivax. Only the ef1-alpha and the vir24 promoters produced detectable luciferase activity. Moreover, these luc activities were two orders of magnitude lower than the ones of the ef1-alpha intergenic regions of P. falciparum and P. berghei. A minimal promoter based on the Pb ef1-alpha promoter was cloned between the Pv ef1-alpha promoter and the luciferase gene. This construction increased about three times the strength of the Pv ef1-alpha promoter. Sequence analysis of the ef1-alpha intergenic regions of six Plasmodium species identified a motif of six nucleotides totally conserved in all of them, but P. vivax. Present efforts are now guided to clone this motif inside the Pv ef1-alpha promoter. Together, these data indicate that promoter regions of P. vivax cannot efficiently recruit the transcription initiation complex of P. falciparum and that both the basal promoter and upstream elements are probably involved in this process
  • Editor: São Paulo Comissão de Cultura e Extensão Universitária do ICB/USP
  • Data de criação/publicação: 2005
  • Formato: [s. p.].
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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