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Genetic Engineering in Mycobacteria

Armianinova, D. K. ; Karpov, D. S. ; Kotliarova, M. S. ; Goncharenko, A. V.

Molecular biology (New York), 2022-12, Vol.56 (6), p.830-841 [Periódico revisado por pares]

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Título:
Genetic Engineering in Mycobacteria
Autor: Armianinova, D. K. ; Karpov, D. S. ; Kotliarova, M. S. ; Goncharenko, A. V.
Assuntos: Bacterial Genome Editing ; Biochemistry ; Biomedical and Life Sciences ; CRISPR ; Genetic engineering ; Genomes ; Growth rate ; Homologous recombination ; Human Genetics ; Leprosy ; Life Sciences ; Tuberculosis ; Virulence
É parte de: Molecular biology (New York), 2022-12, Vol.56 (6), p.830-841
Descrição: Genetic tools for targeted modification of the mycobacterial genome contribute to the understanding of the physiology and virulence mechanisms of mycobacteria. Human and animal pathogens, such as the Mycobacterium tuberculosis complex, which causes tuberculosis, and M. leprae , which causes leprosy, are of particular importance. Genetic research opens up novel opportunities to identify and validate new targets for antibacterial drugs and to develop improved vaccines. Although mycobacteria are difficult to work with due to their slow growth rate and a limited possibility to transfer genetic information, significant progress has been made in developing genetic engineering methods for mycobacteria. The review considers the main approaches to changing the mycobacterial genome in a targeted manner, including homologous and site-specific recombination and use of the CRISPR/Cas system.
Editor: Moscow: Pleiades Publishing
Idioma: Inglês;Russo

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