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Evaluating vaccine-elicited antibody activities against Neisseria gonorrhoeae : cross-protective responses elicited by the 4CMenB meningococcal vaccine

Gray, Mary C ; Thomas, Keena S ; Lamb, Evan R ; Werner, Lacie M ; Connolly, Kristie L ; Jerse, Ann E ; Criss, Alison K Kline, Kimberly A.

Infection and immunity, 2023-12, Vol.91 (12), p.e0030923-e0030923 [Periódico revisado por pares]

United States: American Society for Microbiology

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  • Título:
    Evaluating vaccine-elicited antibody activities against Neisseria gonorrhoeae : cross-protective responses elicited by the 4CMenB meningococcal vaccine
  • Autor: Gray, Mary C ; Thomas, Keena S ; Lamb, Evan R ; Werner, Lacie M ; Connolly, Kristie L ; Jerse, Ann E ; Criss, Alison K
  • Kline, Kimberly A.
  • Assuntos: Animals ; Antibodies ; Antibodies, Bacterial ; Antigens, Bacterial ; Bacterial Vaccines ; Gonorrhea - microbiology ; Humans ; Meningococcal Infections - microbiology ; Meningococcal Vaccines ; Mice ; Microbial Immunity and Vaccines ; Neisseria gonorrhoeae ; Neisseria meningitidis ; Neisseria meningitidis, Serogroup B ; Vaccines ; Vaccines, Combined
  • É parte de: Infection and immunity, 2023-12, Vol.91 (12), p.e0030923-e0030923
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
    Mary C. Gray and Keena S. Thomas contributed equally to this article. Order was determined by seniority.
    Present address: Trauma Infection Research Group, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA
    Present address: Enterics and Sexually Transmitted Infections Branch, Division of Microbiology and Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, USA
    The authors declare no conflict of interest.
  • Descrição: The bacterial pathogen is an urgent global health problem due to increasing numbers of infections, coupled with rampant antibiotic resistance. Vaccines against gonorrhea are being prioritized to combat drug-resistant . Meningococcal serogroup B vaccines such as four-component meningococcal B vaccine (4CMenB) are predicted by epidemiology studies to cross-protect individuals from natural infection with and elicit antibodies that cross-react with . Evaluation of vaccine candidates for gonorrhea requires a suite of assays for predicting efficacy and in animal models of infection, including the role of antibodies elicited by immunization. Here, we present the development and optimization of assays to evaluate antibody functionality after immunization of mice: antibody binding to intact , serum bactericidal activity, and opsonophagocytic killing activity using primary human neutrophils [polymorphonuclear leukocytes (PMNs)]. These assays were developed with purified antibodies against and used to evaluate serum from mice that were vaccinated with 4CMenB or given alum as a negative control. Results from these assays will help prioritize gonorrhea vaccine candidates for advanced preclinical to early clinical studies and will contribute to identifying correlates and mechanisms of immune protection against .
  • Editor: United States: American Society for Microbiology
  • Idioma: Inglês
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