Idioma:

Chitosan-based luminescent/magnetic hybrid nanogels for insulin delivery, cell imaging, and antidiabetic research of dietary supplements

Shen, Jian-Min ; Xu, Luan ; Lu, Yan ; Cao, Hui-Ming ; Xu, Zhi-Gang ; Chen, Tong ; Zhang, Hai-Xia

International journal of pharmaceutics, 2012-05, Vol.427 (2), p.400-409 [Periódico revisado por pares]

Netherlands: Elsevier B.V

Texto completo disponível

Citações Citado por

Enviar para

Título:
Chitosan-based luminescent/magnetic hybrid nanogels for insulin delivery, cell imaging, and antidiabetic research of dietary supplements
Autor: Shen, Jian-Min ; Xu, Luan ; Lu, Yan ; Cao, Hui-Ming ; Xu, Zhi-Gang ; Chen, Tong ; Zhang, Hai-Xia
Assuntos: additives ; Antidiabetic effect ; Catechin - analogs & derivatives ; Catechin - pharmacology ; CdTe quantum dot (CdTe QD) ; Cell Line ; Cell Survival - drug effects ; Chitosan ; Chitosan - chemistry ; Dietary Supplements ; Drug Delivery Systems ; Ferric Compounds - chemistry ; fluorescence ; fluorescence microscopy ; Fourier transform infrared spectroscopy ; gelation ; hepatocytes ; Hepatocytes - metabolism ; Humans ; Hypoglycemic Agents - administration & dosage ; Hypoglycemic Agents - chemistry ; image analysis ; Insulin ; Insulin - administration & dosage ; Insulin - chemistry ; insulin resistance ; iron oxides ; Luminescence ; Magnetics ; Microscopy, Electron, Transmission ; Microscopy, Fluorescence ; Nanogels ; nanomaterials ; Nanoparticles - chemistry ; Particle Size ; Quantum Dots ; Receptor, Insulin - drug effects ; Receptor, Insulin - metabolism ; Spectroscopy, Fourier Transform Infrared ; Superparamagnetic iron oxide ; transmission electron microscopy ; viability ; Xanthones - pharmacology
É parte de: International journal of pharmaceutics, 2012-05, Vol.427 (2), p.400-409
Notas: http://dx.doi.org/10.1016/j.ijpharm.2012.01.059
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: In this work, the chitosan-based luminescent/magnetic (CLM) nanomaterials were synthesized by direct gelation of chitosan, CdTe and superparamagnetic iron oxide into the hybrid nanogels. The morphology, sizes and properties of the nanogels prepared with different chitosan/QD/MNP ratios and under different processing parameters were researched. Fluorescence microscopy, FTIR spectra and TEM images confirmed the success of the preparation of the CLM hybrid nanogels. Spherical CLM hybrid nanogels with appropriate average sizes (<160nm) were used for insulin loading. The actual loading amount of insulin was approximately 40.1mg/g. Human normal hepatocytes L02 cell line was used to explore the effects of additives, such as mangiferin (MF), (−)-epigallocatechin gallate (EGCG), and (−)-epicatechin gallate (ECG) on the insulin-receptor-mediated cellular uptake using insulin-loaded CLM (ICLM) hybrid nanogels. Above 80% of viability of L02 cells were watched at a nanogels concentration of 500μg/mL whatever the additives existed or not. The study discovered that the fluorescent signals of the ICLM hybrid nanogels in L02 cells were more intense in the presence of MF, EGCG and ECG in medium than in the absence of these components, respectively. These results demonstrate that MF, EGCG and ECG are potentially able to enhance targeting combination of insulin with L02 cells and improve insulin sensitivity in L02 cells. The hybrid nanogels designed as a targeting carrier can potentially offer an approach for integration of insulin delivery, cell imaging, and antidiabetic investigation of dietary supplements.
Editor: Netherlands: Elsevier B.V
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

Chitosan-based luminescent/magnetic hybrid nanogels for insulin delivery, cell imaging, and antidiabetic research of dietary supplements

Shen, Jian-Min ; Xu, Luan ; Lu, Yan ; Cao, Hui-Ming ; Xu, Zhi-Gang ; Chen, Tong ; Zhang, Hai-Xia

Netherlands: Elsevier B.V

Buscando em bases de dados remotas. Favor aguardar.