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Clinical and Environmental Isolates of Legionella pneumophila Serogroup 1 Cannot Be Distinguished by Sequence Analysis of Two Surface Protein Genes and Three Housekeeping Genes

Aurell, Helena ; Farge, Pierre ; Meugnier, Hélène ; Gouy, Manolo ; Forey, Françoise ; Lina, Gérard ; Vandenesch, François ; Etienne, Jerome ; Jarraud, Sophie

Applied and Environmental Microbiology, 2005, Vol.71 (1), p.282-289 [Periódico revisado por pares]

Washington, DC: American Society for Microbiology

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Título:
Clinical and Environmental Isolates of Legionella pneumophila Serogroup 1 Cannot Be Distinguished by Sequence Analysis of Two Surface Protein Genes and Three Housekeeping Genes
Autor: Aurell, Helena ; Farge, Pierre ; Meugnier, Hélène ; Gouy, Manolo ; Forey, Françoise ; Lina, Gérard ; Vandenesch, François ; Etienne, Jerome ; Jarraud, Sophie
Assuntos: Analysis ; Bacteria ; Bacterial Proteins - chemistry ; Bacterial Proteins - genetics ; Bacterial Typing Techniques ; Bacteriology ; Base Sequence ; Biological and medical sciences ; Fundamental and applied biological sciences. Psychology ; Genes ; Hospitals ; Humans ; Legionella pneumophila ; Legionella pneumophila - classification ; Legionella pneumophila - genetics ; Legionella pneumophila - isolation & purification ; Legionnaires' Disease - microbiology ; Life Sciences ; Microbiology ; Molecular Sequence Data ; Other ; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains ; Phylogeny ; Proteins ; Public Health Microbiology ; Sequence Analysis, DNA ; Water Microbiology ; Water Supply
É parte de: Applied and Environmental Microbiology, 2005, Vol.71 (1), p.282-289
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PMCID: PMC544207
Corresponding author. Mailing address: Centre National de Référence des Legionella, INSERM E-0230, Laboratoire de Bactériologie, Faculté de Médecine Laennec IFR 62, 7 rue Guillaume Paradin, 69372 Lyon cedex 08, France. Phone: 33 4 78 77 86 57. Fax: 33 4 78 77 86 58. E-mail: sophie.jarraud@univ-lyon1.fr.
Descrição: We used gene sequencing to determine whether clinical (sporadic, epidemic, and endemic) and environmental isolates of Legionella pneumophila serogroup (sg) 1 belong to specific lineages. A total of 178 clinical and environmental L. pneumophila sg 1 isolates, defined by pulsed-field gel electrophoresis and epidemiological data as sporadic, epidemic, or endemic, were analyzed for polymorphisms in five gene fragments. The fragments belonged to three housekeeping genes (coding for aconitase [acn], aspartate-[beta]-semialdehyde dehydrogenase [asd], and RNA polymerase [beta] subunit [rpoB]) and two surface protein genes (coding for the macrophage infectivity potentiator [mip] and the major outer membrane protein [mompS]). The phylogenetic tree inferred from sequence polymorphisms of the five genes identified two large clusters, one consisting of 133 poorly differentiated strains and containing two smaller clusters (10 and 2 strains) unrelated to each other and the other consisting of 42 strains. Clinical and environmental isolates could not be distinguished on this basis, and no link between genetic background and epidemiological type was found, suggesting that other factors are responsible for differences in pathogenicity.
Editor: Washington, DC: American Society for Microbiology
Idioma: Inglês

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Clinical and Environmental Isolates of Legionella pneumophila Serogroup 1 Cannot Be Distinguished by Sequence Analysis of Two Surface Protein Genes and Three Housekeeping Genes

Aurell, Helena ; Farge, Pierre ; Meugnier, Hélène ; Gouy, Manolo ; Forey, Françoise ; Lina, Gérard ; Vandenesch, François ; Etienne, Jerome ; Jarraud, Sophie

Washington, DC: American Society for Microbiology

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