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Multiplex-PCR Method for Species Identification of Coagulase-Positive Staphylococci

Sasaki, Takashi ; Tsubakishita, Sae ; Tanaka, Yoshikazu ; Sakusabe, Arihito ; Ohtsuka, Masayuki ; Hirotaki, Shintaro ; Kawakami, Tetsuji ; Fukata, Tsuneo ; Hiramatsu, Keiichi

Journal of Clinical Microbiology, 2010-03, Vol.48 (3), p.765-769 [Periódico revisado por pares]

Washington, DC: American Society for Microbiology

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Título:
Multiplex-PCR Method for Species Identification of Coagulase-Positive Staphylococci
Autor: Sasaki, Takashi ; Tsubakishita, Sae ; Tanaka, Yoshikazu ; Sakusabe, Arihito ; Ohtsuka, Masayuki ; Hirotaki, Shintaro ; Kawakami, Tetsuji ; Fukata, Tsuneo ; Hiramatsu, Keiichi
Assuntos: Animals ; Bacteriological Techniques - methods ; Bacteriology ; Biological and medical sciences ; DNA Primers - genetics ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; Fundamental and applied biological sciences. Psychology ; Microbiology ; Micrococcal Nuclease - genetics ; Miscellaneous ; Molecular Sequence Data ; Polymerase Chain Reaction - methods ; Polymorphism, Genetic ; Sensitivity and Specificity ; Staphylococcal Infections - diagnosis ; Staphylococcal Infections - microbiology ; Staphylococcal Infections - veterinary ; Staphylococcus - classification ; Staphylococcus - genetics ; Staphylococcus - isolation & purification ; Staphylococcus aureus ; Veterinary Medicine - methods
É parte de: Journal of Clinical Microbiology, 2010-03, Vol.48 (3), p.765-769
Notas: ObjectType-Article-2
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Descrição: In veterinary medicine, coagulase-positive staphylococci (CoPS) other than Staphylococcus aureus have frequently been misidentified as being S. aureus strains, as they have several phenotypic traits in common. There has been no reliable method to distinguish among CoPS species in veterinary clinical laboratories. In the present study, we sequenced the thermonuclease (nuc) genes of staphylococcal species and devised a multiplex-PCR (M-PCR) method for species identification of CoPS by targeting the nuc gene locus. To evaluate sensitivity and specificity, we used this M-PCR method on 374 staphylococcal strains that had been previously identified to the species level by an hsp60 sequencing approach. We could successfully distinguish between S. aureus, S. hyicus, S. schleiferi, S. intermedius, S. pseudintermedius, and S. delphini groups A and B. The present method was both sensitive (99.8%) and specific (100%). Our M-PCR assay will allow the routine species identification of CoPS isolates from various animal species for clinical veterinary diagnosis.
Editor: Washington, DC: American Society for Microbiology
Idioma: Inglês

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Multiplex-PCR Method for Species Identification of Coagulase-Positive Staphylococci

Sasaki, Takashi ; Tsubakishita, Sae ; Tanaka, Yoshikazu ; Sakusabe, Arihito ; Ohtsuka, Masayuki ; Hirotaki, Shintaro ; Kawakami, Tetsuji ; Fukata, Tsuneo ; Hiramatsu, Keiichi

Washington, DC: American Society for Microbiology

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