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Activation of PKC results in improved contractile effects and Ca2+ cycling by inhibition of PP2A-B56α

Pluteanu, Florentina ; Boknik, Peter ; Heinick, Alexander ; König, Christiane ; Müller, Frank U ; Weidlich, Adam ; Kirchhefer, Uwe

American journal of physiology. Heart and circulatory physiology, 2022-03, Vol.322 (3), p.H427-H441 [Peer Reviewed Journal]

Bethesda: American Physiological Society

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Title:
Activation of PKC results in improved contractile effects and Ca2+ cycling by inhibition of PP2A-B56α
Author: Pluteanu, Florentina ; Boknik, Peter ; Heinick, Alexander ; König, Christiane ; Müller, Frank U ; Weidlich, Adam ; Kirchhefer, Uwe
Subjects: Acetic acid ; Calcium (intracellular) ; Calcium channels (L-type) ; Calcium ions ; Calcium signalling ; Cardiomyocytes ; Cycles ; Dephosphorylation ; Genotypes ; Inactivation ; Intracellular signalling ; Muscle contraction ; Myocytes ; Myosin ; Myosin-binding protein C ; Phorbol 12-myristate 13-acetate ; Phosphoprotein phosphatase ; Phosphorylation ; Protein C ; Protein kinase C ; Protein phosphatase ; Proteins ; Transgenic mice
Is Part Of: American journal of physiology. Heart and circulatory physiology, 2022-03, Vol.322 (3), p.H427-H441
Notes: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Description: Protein phosphatase 2A (PP2A) represents a heterotrimer that is responsible for the dephosphorylation of important regulatory myocardial proteins. This study was aimed to test whether the phosphorylation of PP2A-B56α at Ser41 by PKC is involved in the regulation of myocyte Ca2+ cycling and contraction. For this purpose, heart preparations of wild-type (WT) and transgenic mice overexpressing the nonphosphorylatable S41A mutant form (TG) were stimulated by administration of the direct PKC activator phorbol 12-myristate 13-acetate (PMA), and functional effects were studied. PKC activation was accompanied by the inhibition of PP2A activity in WT cardiomyocytes, whereas this effect was absent in TG. Consistently, the increase in the sarcomere length shortening and the peak amplitude of Ca2+ transients after PMA administration in WT cardiomyocytes was attenuated in TG. However, the costimulation with 1 µM isoprenaline was able to offset these functional deficits. Moreover, TG hearts did not show an increase in the phosphorylation of the myosin-binding protein C after administration of PMA but was detected in corresponding WT. PMA modulated voltage-dependent activation of the L-type Ca2+ channel (LTCC) differently in the two genotypes, shifting V1/2a by +1.5 mV in TG and by −2.4 mV in WT. In the presence of PMA, ICaL inactivation remained unchanged in TG, whereas it was slower in corresponding WT. Our data suggest that PKC-activated enhancement of myocyte contraction and intracellular Ca2+ signaling is mediated by phosphorylation of B56α at Ser41, leading to a decrease in PP2A activity.
Publisher: Bethesda: American Physiological Society
Language: English

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Activation of PKC results in improved contractile effects and Ca2+ cycling by inhibition of PP2A-B56α

Pluteanu, Florentina ; Boknik, Peter ; Heinick, Alexander ; König, Christiane ; Müller, Frank U ; Weidlich, Adam ; Kirchhefer, Uwe

Bethesda: American Physiological Society

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