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Interaction of calmodulin with synaptic plasma membranes isolated from sheep brain

Lopes, M.C.F. ; Vale, M.G.P.

Neurochemistry international, 1989, Vol.14 (4), p.455-459 [Periódico revisado por pares]

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Título:
Interaction of calmodulin with synaptic plasma membranes isolated from sheep brain
Autor: Lopes, M.C.F. ; Vale, M.G.P.
Assuntos: Biochemistry and metabolism ; Biological and medical sciences ; brain ; Central nervous system ; Fundamental and applied biological sciences. Psychology ; synaptic membranes ; Vertebrates: nervous system and sense organs
É parte de: Neurochemistry international, 1989, Vol.14 (4), p.455-459
Notas: ObjectType-Article-2
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Descrição: Calmodulin binding to a membrane fraction enriched in synaptic plasma membranes of sheep brain cells was investigated with [ 125I]calmodulin. Calmodulin binding to these membranes is Ca 2+-dependent with a half maximal saturation at the pCa value of about 5.5. The binding is reduced by replacing Ca 2+ with Mg 2+, but it is significantly enhanced when both cations are present in the medium. Cation-dependent binding is specific and saturable with an apparent K D of about 47–50 nM and a maximal capacity of about 4 pmol mg −1 protein. The results indicate that synaptic plasma membranes isolated from sheep brain cells interact with calmodulin in a Ca 2+-dependent, Mg 2+-facilitated manner.
Editor: Oxford: Elsevier Ltd
Idioma: Inglês

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