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Assessing the taxonomic status of Osmoderma cristinae (Coleoptera: Scarabaeidae), endemic to Sicily, by genetic, morphological and pheromonal analyses

Zauli, Agnese ; Carpaneto, Giuseppe M. ; Chiari, Stefano ; Mancini, Emiliano ; Nyabuga, Franklin N. ; Redolfi De Zan, Lara ; Romiti, Federico ; Sabbani, Sunil ; Audisio, Paolo A. ; Hedenström, Erik ; Bologna, Marco A. ; Svensson, Glenn P.

Journal of zoological systematics and evolutionary research, 2016-08, Vol.54 (3), p.206-214 [Periódico revisado por pares]

Berlin: Blackwell Publishing Ltd

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  • Título:
    Assessing the taxonomic status of Osmoderma cristinae (Coleoptera: Scarabaeidae), endemic to Sicily, by genetic, morphological and pheromonal analyses
  • Autor: Zauli, Agnese ; Carpaneto, Giuseppe M. ; Chiari, Stefano ; Mancini, Emiliano ; Nyabuga, Franklin N. ; Redolfi De Zan, Lara ; Romiti, Federico ; Sabbani, Sunil ; Audisio, Paolo A. ; Hedenström, Erik ; Bologna, Marco A. ; Svensson, Glenn P.
  • Assuntos: Amplified fragment length polymorphism ; Biochemistry and Molecular Biology ; Biokemi och molekylärbiologi ; Biologi ; Biological Sciences ; Biological Systematics ; Biologisk systematik ; C oxidase I gene ; Coleoptera ; Enantiomeric specificity ; Geometric morphometrics ; Natural Sciences ; Naturvetenskap ; Saproxylic beetles ; Scarabaeidae
  • É parte de: Journal of zoological systematics and evolutionary research, 2016-08, Vol.54 (3), p.206-214
  • Notas: istex:6D84C906006E18E126A60F1818F6E8EE88DFA37D
    ArticleID:JZS12127
    LIFE11 - No. NAT/IT/000252 MIPP
    Table S1. AFLP primer names and sequences. Appendix S2. Synthetic path for the 100% enantiomerically pure (S)-(-)-γ-decalactone. Figure S3. Segregating sites of the mtDNA cytochrome C oxidase I gene (COI) in Osmoderma cristinae and Osmoderma eremita. Figure S4. Additional pictures of specimens studied for the morphological analysis of Osmoderma cristinae. Figure S5. Additional pictures of specimens studied for the morphological analysis of Osmoderma eremita. Figure S6. Landmarks (black dots) and semilandmarks (white dots) configuration on Osmoderma cristinae (A and B) and O. eremita (C and D) paramere in dorsal (A and C) and lateral (B and D) view (scale bar = 1 mm).
    ark:/67375/WNG-S19V6WSX-W
    ObjectType-Article-1
    SourceType-Scholarly Journals-1
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  • Descrição: Resolving complexes of closely related and cryptic insect species can be challenging, especially when dealing with rare and protected taxa that are difficult to collect for genetic and morphological analyses. Until recently, populations of the genus Osmoderma (Scarabaeidae), widespread in Europe, were treated as a single species O. eremita (Scopoli, 1763) in spite of observed geographic variation in morphology. A previous survey using sequence data from the mtDNA cytochrome C oxidase I gene (COI) revealed the occurrence of at least two distinct lineages within this species complex: O. eremita in the west and O. barnabita Motschulsky, 1845, in the east. Interestingly, beetles confined to Sicily have been described as a distinct species, O. cristinae Sparacio, 1994, based on morphological traits. Only few Sicilian specimens were included in the former genetic analysis, and the results led to a still questionable taxonomic rank for these populations. To explore the robustness of the previous taxonomic arrangement for O. cristinae, a combination of genetic, morphological and pheromonal analyses was used. A 617‐bp fragment of the COI gene, aligned with O. cristinae and O. eremita sequences already available in GenBank, showed a clear genetic divergence between the two species (interspecific mean distance = 6.6%). Moreover, results from AFLP markers sustained the distinction of the two species. In addition, geometric morphometric analyses of the shape of male genitalia revealed a clear differentiation between the two species. Via scent analysis and field trapping, we demonstrated the production of the sex pheromone (R)‐(+)‐γ‐decalactone by males of O. cristinae, the attraction by conspecific individuals (mostly females) to this compound, and a lack of antagonistic effect of (S)‐(–)‐γ‐decalactone. The fact that O. eremita and O. eremita use the same compound for mate finding suggests that this sex pheromone has not undergone a differentiation and probably the allopatry of these two species compensates for the absence of a mechanism to avoid cross‐attraction. Our genetic and morphological data support the divergence of the two species and confirm the species status for O. cristinae, while sex pheromones are confirmed to be invariant among different species of the genus Osmoderma. Our molecular analyses based on the COI gene and AFLP support a clear genetic divergence between the scarab beetle Osmoderma cristinae (endemic to Sicily) and the closely related O. eremita. Moreover, geometric morphometric analyses of male genitalia reveal a differentiation between the two species. Nevertheless, the sex pheromone used by these species is the same, suggesting that this compound has not undergone a differentiation.
  • Editor: Berlin: Blackwell Publishing Ltd
  • Idioma: Inglês
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