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Presence of HHV-6A in Endometrial Epithelial Cells from Women with Primary Unexplained Infertility

Marci, Roberto ; Gentili, Valentina ; Bortolotti, Daria ; Lo Monte, Giuseppe ; Caselli, Elisabetta ; Bolzani, Silvia ; Rotola, Antonella ; Di Luca, Dario ; Rizzo, Roberta Jeong, Jae-Wook

PloS one, 2016-07, Vol.11 (7), p.e0158304-e0158304 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Presence of HHV-6A in Endometrial Epithelial Cells from Women with Primary Unexplained Infertility
Autor: Marci, Roberto ; Gentili, Valentina ; Bortolotti, Daria ; Lo Monte, Giuseppe ; Caselli, Elisabetta ; Bolzani, Silvia ; Rotola, Antonella ; Di Luca, Dario ; Rizzo, Roberta
Jeong, Jae-Wook
Assuntos: Adult ; Biology and life sciences ; Biopsy ; Causes of ; Cytokines ; Cytokines - secretion ; Deoxyribonucleic acid ; DNA ; Endometrium ; Endometrium - pathology ; Epithelial cells ; Epithelial Cells - virology ; Ethics ; Female ; Female infertility ; Flushing ; Herpesviridae ; Herpesvirus 6, Human - isolation & purification ; Herpesvirus 6, Human - physiology ; Human herpesvirus 6 ; Human herpesvirus 6B ; Humans ; Immune response ; Infections ; Infertility ; Infertility, Female ; Infertility, Female - immunology ; Infertility, Female - metabolism ; Infertility, Female - pathology ; Infertility, Female - virology ; Interferon ; Interleukin 10 ; Killer Cells, Natural - immunology ; Medicine and Health Sciences ; Menstruation ; Natural killer cells ; Phenotype ; Phenotypes ; Physiological aspects ; Pregnancy ; Uterus ; Uterus - secretion ; Viruses ; Womens health ; Young Adult
É parte de: PloS one, 2016-07, Vol.11 (7), p.e0158304-e0158304
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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Competing Interests: The authors have declared that no competing interests exist.
Conceived and designed the experiments: RR RM DDL. Performed the experiments: VG DB EC SB. Analyzed the data: AR RR. Contributed reagents/materials/analysis tools: GLM. Wrote the paper: RR RM DDL.
Descrição: To elucidate the roles of human herpesvirus (HHV)-6 primary unexplained infertile women, a prospective randomized study was conducted on a cohort of primary unexplained infertile women and a cohort of control women, with at least one successful pregnancy. HHV-6 DNA was analyzed and the percentage and immune-phenotype of resident endometrial Natural Killer (NK) cells, as the first line of defense towards viral infections, was evaluated in endometrial biopsies. Cytokine levels in uterine flushing samples were analyzed. HHV-6A DNA was found in 43% of endometrial biopsies from primary unexplained infertile women, but not in control women. On the contrary, HHV-6B DNA was absent in endometrial biopsies, but present in PBMCs of both cohorts. Endometrial NK cells presented a different distribution in infertile women with HHV6-A infection compared with infertile women without HHV6-A infection. Notably, we observed a lower percentage of endometrial specific CD56brightCD16- NK cells. We observed an enhanced HHV-6A-specific endometrial NK cell response in HHV-6A positive infertile women, with a marked increase in the number of endometrial NK cells activating towards HHV-6A infected cells. The analysis of uterine flushing samples showed an increase in IL-10 levels and a decrease of IFN-gamma concentrations in infertile women with HHV6-A infection. Our study indicates, for the first time, that HHV-6A infection might be an important factor in female unexplained infertility development, with a possible role in modifying endometrial NK cells immune profile and ability to sustain a successful pregnancy.
Editor: United States: Public Library of Science
Idioma: Inglês

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Presence of HHV-6A in Endometrial Epithelial Cells from Women with Primary Unexplained Infertility

Marci, Roberto ; Gentili, Valentina ; Bortolotti, Daria ; Lo Monte, Giuseppe ; Caselli, Elisabetta ; Bolzani, Silvia ; Rotola, Antonella ; Di Luca, Dario ; Rizzo, Roberta Jeong, Jae-Wook

United States: Public Library of Science

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