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Effect of Soil Type and Fertilizer Management on Archaeal Community in Upland Field Soils

Hoshino, Yuko Takada ; Morimoto, Sho ; Hayatsu, Masahito ; Nagaoka, Kazunari ; Suzuki, Chika ; Karasawa, Toshihiko ; Takenaka, Makoto ; Akiyama, Hiroko

Microbes and Environments, 2011, Vol.26(4), pp.307-316

Japan: Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles

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Título:
Effect of Soil Type and Fertilizer Management on Archaeal Community in Upland Field Soils
Autor: Hoshino, Yuko Takada ; Morimoto, Sho ; Hayatsu, Masahito ; Nagaoka, Kazunari ; Suzuki, Chika ; Karasawa, Toshihiko ; Takenaka, Makoto ; Akiyama, Hiroko
Assuntos: 16S rRNA gene ; Animals ; Archaea ; Archaea - classification ; Archaea - genetics ; Archaea - isolation & purification ; archaeal community ; Biota ; Cluster Analysis ; copy number ; Crenarchaeota ; Denaturing Gradient Gel Electrophoresis ; DNA, Archaeal - chemistry ; DNA, Archaeal - genetics ; DNA, Ribosomal - chemistry ; DNA, Ribosomal - genetics ; Fertilizers ; Fertilizers - statistics & numerical data ; Gel electrophoresis ; Manure ; Molecular Sequence Data ; Nitrogen - analysis ; Oryza sativa ; PCR-DGGE ; Phylogeny ; Polymerase Chain Reaction ; real-time PCR ; RNA, Ribosomal, 16S - genetics ; rRNA 16S ; Ruminants ; Sequence Analysis, DNA ; Soil - analysis ; Soil Microbiology ; soil nitrogen cycle ; Soil types ; Swine
É parte de: Microbes and Environments, 2011, Vol.26(4), pp.307-316
Notas: ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
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Descrição: The effects of soil and fertilizer types on archaeal communities were evaluated by real-time PCR and PCR-denaturing gradient gel electrophoresis (DGGE) targeting the 16S rRNA gene of total DNA directly extracted from upland field soils. Twelve experimental upland field plots containing four different soil types, i.e., Cumulic Andosol, Low-humic Andosol, Yellow Soil and Gray Lowland Soil, were maintained under three different fertilizer management systems for 8 years (chemical fertilizer, rice husks and cow manure, and pig manure, respectively). Two-way ANOVA and RDA analyses showed that the copy number and PCR-DGGE profile of archaeal 16S rRNA gene were affected mainly by soil type, especially between Andosol and non-Andosol, but were also influenced by fertilizer type. Among several soil chemical properties, total N content showed a significant correlation to archaeal community. Sequence analyses showed that most of the major DGGE bands corresponded to uncultured Crenarchaeota of Group I.1b that contained ammonia-oxidizing archaea (AOA). These sequences were separated into two clusters in the phylogenetic tree and each lineage showed a different response to total N content.
Editor: Japan: Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles
Idioma: Inglês

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Effect of Soil Type and Fertilizer Management on Archaeal Community in Upland Field Soils

Hoshino, Yuko Takada ; Morimoto, Sho ; Hayatsu, Masahito ; Nagaoka, Kazunari ; Suzuki, Chika ; Karasawa, Toshihiko ; Takenaka, Makoto ; Akiyama, Hiroko

Japan: Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles

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