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Periplocoside E, an Effective Compound from Periploca sepium Bge, Inhibited T Cell Activation in Vitro and in Vivo

Zhu, Yi-Na ; Zhao, Wei-Min ; Yang, Yi-Fu ; Liu, Qun-Fang ; Zhou, Yu ; Tian, Jia ; Ni, Jia ; Fu, Yun-Feng ; Zhong, Xiang-Gen ; Tang, Wei ; Zhou, Ru ; He, Pei-Lan ; Li, Xiao-Yu ; Zuo, Jian-Ping

The Journal of pharmacology and experimental therapeutics, 2006-02, Vol.316 (2), p.662-669 [Periódico revisado por pares]

United States: American Society for Pharmacology and Experimental Therapeutics

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Título:
Periplocoside E, an Effective Compound from Periploca sepium Bge, Inhibited T Cell Activation in Vitro and in Vivo
Autor: Zhu, Yi-Na ; Zhao, Wei-Min ; Yang, Yi-Fu ; Liu, Qun-Fang ; Zhou, Yu ; Tian, Jia ; Ni, Jia ; Fu, Yun-Feng ; Zhong, Xiang-Gen ; Tang, Wei ; Zhou, Ru ; He, Pei-Lan ; Li, Xiao-Yu ; Zuo, Jian-Ping
Assuntos: Animals ; Antigen-Presenting Cells - drug effects ; Antigen-Presenting Cells - immunology ; Cell Proliferation - drug effects ; Cell Survival - drug effects ; Cells, Cultured ; Cytokines - immunology ; Drugs, Chinese Herbal - isolation & purification ; Drugs, Chinese Herbal - pharmacology ; Drugs, Chinese Herbal - therapeutic use ; Female ; Hypersensitivity, Delayed - drug therapy ; Hypersensitivity, Delayed - immunology ; Immunosuppressive Agents - isolation & purification ; Immunosuppressive Agents - pharmacology ; Immunosuppressive Agents - therapeutic use ; Lymphocyte Activation - drug effects ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Oligosaccharides - isolation & purification ; Oligosaccharides - pharmacology ; Oligosaccharides - therapeutic use ; Ovalbumin - immunology ; Periploca - chemistry ; Plant Bark - chemistry ; Pregnenes - isolation & purification ; Pregnenes - pharmacology ; Pregnenes - therapeutic use ; T-Lymphocytes - drug effects ; T-Lymphocytes - immunology
É parte de: The Journal of pharmacology and experimental therapeutics, 2006-02, Vol.316 (2), p.662-669
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Periploca sepium Bge, a traditional Chinese herb medicine, is used for treating rheumatoid arthritis in China. Followed the bioactivity-guided isolation, the most potent immunosuppressive compound, periplocoside E (PSE), a pregnane glycoside, had been identified from P. sepium Bge. We investigated the immunosuppressive effects of PSE in vitro and in vivo. The results showed that PSE in a dose-dependent manner significantly inhibited the proliferation of splenocytes induced by concanavalin A and mixed lymphocyte culture reaction at no cytotoxic concentrations (<5 Î¼M). Administration of PSE suppressed a delayed-type hypersensitivity reaction, and ovalbumin (OVA) induced antigen-specific immune responses in mice. In vivo treatment with PSE dose dependently suppressed OVA-induced proliferation and cytokine [interleukin (IL)-2 and interferon (IFN)-Î³] production from splenocytes in vitro. Purified T cells from OVA-immunized mice with PSE treatment showed its low ability for activation by OVA plus normal antigen presenting cell stimulation again in vitro. Further studies showed PSE dose dependently inhibited anti-CD3-induced primary T cell proliferation, activation for IL-2RÎ± (CD25) expression, and cytokine (IFN-Î³ and IL-2) production also at the transcriptional level. PSE was highly specific and significantly inhibited the activation of extracellular signal-regulated kinase and Jun N-terminal kinase, whereas activation of p38 was not affected in T cells stimulated with anti-CD3. These results demonstrated that PSE is an immunosuppressive compound in P. sepium Bge, which directly inhibits T cell activation in vitro and in vivo. This study provided evidence to understand the therapeutic effects of P. sepium Bge and indicated that this herb is appropriate for treatment of T cell-mediated disorders, such as autoimmune diseases.
Editor: United States: American Society for Pharmacology and Experimental Therapeutics
Idioma: Inglês

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Periplocoside E, an Effective Compound from Periploca sepium Bge, Inhibited T Cell Activation in Vitro and in Vivo

Zhu, Yi-Na ; Zhao, Wei-Min ; Yang, Yi-Fu ; Liu, Qun-Fang ; Zhou, Yu ; Tian, Jia ; Ni, Jia ; Fu, Yun-Feng ; Zhong, Xiang-Gen ; Tang, Wei ; Zhou, Ru ; He, Pei-Lan ; Li, Xiao-Yu ; Zuo, Jian-Ping

United States: American Society for Pharmacology and Experimental Therapeutics

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