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Experimental study on the action of allitridin against human cytomegalovirus in vitro: Inhibitory effects on immediate-early genes

Zhen, Hong ; Fang, Feng ; Ye, Du-yun ; Shu, Sai-nan ; Zhou, Yu-feng ; Dong, Yong-sui ; Nie, Xing-cao ; Li, Ge

Antiviral research, 2006-10, Vol.72 (1), p.68-74 [Periódico revisado por pares]

Amsterdam: Elsevier B.V

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Título:
Experimental study on the action of allitridin against human cytomegalovirus in vitro: Inhibitory effects on immediate-early genes
Autor: Zhen, Hong ; Fang, Feng ; Ye, Du-yun ; Shu, Sai-nan ; Zhou, Yu-feng ; Dong, Yong-sui ; Nie, Xing-cao ; Li, Ge
Assuntos: Allium sativum ; Allyl Compounds - pharmacology ; Antibiotics. Antiinfectious agents. Antiparasitic agents ; Antiviral agents ; Antiviral Agents - pharmacology ; Biological and medical sciences ; Cell Line ; Cytomegalovirus ; Cytomegalovirus - drug effects ; Cytomegalovirus - genetics ; Cytomegalovirus - physiology ; Diallyl trisulfide ; Dose-Response Relationship, Drug ; Gene Expression - drug effects ; General pharmacology ; Genes, Immediate-Early - drug effects ; Genes, Viral - drug effects ; Human cytomegalovirus ; Humans ; Immediate-early gene ; Immediate-Early Proteins - genetics ; Medical sciences ; Pharmacognosy. Homeopathy. Health food ; Pharmacology. Drug treatments ; Sulfides - pharmacology ; Trans-Activators - genetics ; Transcription, Genetic - drug effects ; Viral Proteins - genetics ; Virus Replication - drug effects
É parte de: Antiviral research, 2006-10, Vol.72 (1), p.68-74
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: Garlic ( Allium sativum) extraction has been reported having anti-HCMV efficacy. This study was aimed to investigate the effect of allitridin (diallyl trisulfide, a compound from A. sativum extraction) on the replication of HCMV and the expression of viral immediate-early genes. In HCMV plaque-reduction assay, allitridin appeared a dose-dependent inhibitory ability with EC 50 value of 4.2 μg/ml (selective index, SI = 16.7). Time-of-addition and time-of-removal studies showed that allitridin inhibited HCMV replication in earlier period of viral cycle before viral DNA synthesis. Both immediate early gene (ie1) transcription and IEA (IE 172 and IE 286) expression was suppressed by allitridin, but not by GCV in a single HCMV cycle format. In addition, allitridin appeared stronger inhibition on IE 286 than on IE 172. Decrease of viral DNA load in infected cells was also detected under allitridin treatment, probably due to an indirect consequence of the reduction in ie gene transcription. In summary, this study indicated that allitridin has anti-HCMV activity and the mechanism is associated with suppression of ie gene transcription.
Editor: Amsterdam: Elsevier B.V
Idioma: Inglês

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Experimental study on the action of allitridin against human cytomegalovirus in vitro: Inhibitory effects on immediate-early genes

Zhen, Hong ; Fang, Feng ; Ye, Du-yun ; Shu, Sai-nan ; Zhou, Yu-feng ; Dong, Yong-sui ; Nie, Xing-cao ; Li, Ge

Amsterdam: Elsevier B.V

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