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Lycopene β-cyclase expression influences plant physiology, development, and metabolism in tobacco plants

Kössler, Stella ; Armarego-Marriott, Tegan ; Tarkowská, Danuše ; Turečková, Veronika ; Agrawal, Shreya ; Mi, Jianing ; de Souza, Leonardo Perez ; Schöttler, Mark Aurel ; Schadach, Anne ; Fröhlich, Anja ; Bock, Ralph ; Al-Babili, Salim ; Ruf, Stephanie ; Sampathkumar, Arun ; Moreno, Juan C Dodd, Ian

Journal of experimental botany, 2021-03, Vol.72 (7), p.2544-2569 [Periódico revisado por pares]

UK: Oxford University Press

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Título:
Lycopene β-cyclase expression influences plant physiology, development, and metabolism in tobacco plants
Autor: Kössler, Stella ; Armarego-Marriott, Tegan ; Tarkowská, Danuše ; Turečková, Veronika ; Agrawal, Shreya ; Mi, Jianing ; de Souza, Leonardo Perez ; Schöttler, Mark Aurel ; Schadach, Anne ; Fröhlich, Anja ; Bock, Ralph ; Al-Babili, Salim ; Ruf, Stephanie ; Sampathkumar, Arun ; Moreno, Juan C
Dodd, Ian
Assuntos: Carotenoids ; Gene Expression Regulation, Plant ; Intramolecular Lyases - genetics ; Intramolecular Lyases - metabolism ; Nicotiana - genetics ; Nicotiana - metabolism ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plants, Genetically Modified - genetics ; Plants, Genetically Modified - metabolism ; Research Papers
É parte de: Journal of experimental botany, 2021-03, Vol.72 (7), p.2544-2569
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Abstract Carotenoids are important isoprenoids produced in the plastids of photosynthetic organisms that play key roles in photoprotection and antioxidative processes. β-Carotene is generated from lycopene by lycopene β-cyclase (LCYB). Previously, we demonstrated that the introduction of the Daucus carota (carrot) DcLCYB1 gene into tobacco (cv. Xanthi) resulted in increased levels of abscisic acid (ABA) and especially gibberellins (GAs), resulting in increased plant yield. In order to understand this phenomenon prior to exporting this genetic strategy to crops, we generated tobacco (Nicotiana tabacum cv. Petit Havana) mutants that exhibited a wide range of LCYB expression. Transplastomic plants expressing DcLCYB1 at high levels showed a wild-type-like growth, even though their pigment content was increased and their leaf GA1 content was reduced. RNA interference (RNAi) NtLCYB lines showed different reductions in NtLCYB transcript abundance, correlating with reduced pigment content and plant variegation. Photosynthesis (leaf absorptance, Fv/Fm, and light-saturated capacity of linear electron transport) and plant growth were impaired. Remarkably, drastic changes in phytohormone content also occurred in the RNAi lines. However, external application of phytohormones was not sufficient to rescue these phenotypes, suggesting that altered photosynthetic efficiency might be another important factor explaining their reduced biomass. These results show that LCYB expression influences plant biomass by different mechanisms and suggests thresholds for LCYB expression levels that might be beneficial or detrimental for plant growth. A lycopene β-cyclase expression threshold influences plant biomass, development, and metabolism by modulating pigment and phytohormone content in transplastomic and RNAi tobacco lines.
Editor: UK: Oxford University Press
Idioma: Inglês

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Lycopene β-cyclase expression influences plant physiology, development, and metabolism in tobacco plants

UK: Oxford University Press

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