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Absence of CD47 in protein 4.2–deficient hereditary spherocytosis in man: an interaction between the Rh complex and the band 3 complex

Bruce, Lesley J. ; Ghosh, Sandip ; King, May Jean ; Layton, D. Mark ; Mawby, William J. ; Stewart, Gordon W. ; Oldenborg, Per-Arne ; Delaunay, Jean ; Tanner, Michael J.A.

Blood, 2002-09, Vol.100 (5), p.1878-1885 [Periódico revisado por pares]

Washington, DC: Elsevier Inc

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Título:
Absence of CD47 in protein 4.2–deficient hereditary spherocytosis in man: an interaction between the Rh complex and the band 3 complex
Autor: Bruce, Lesley J. ; Ghosh, Sandip ; King, May Jean ; Layton, D. Mark ; Mawby, William J. ; Stewart, Gordon W. ; Oldenborg, Per-Arne ; Delaunay, Jean ; Tanner, Michael J.A.
Assuntos: Adult ; Anemias. Hemoglobinopathies ; Anion Exchange Protein 1, Erythrocyte - metabolism ; Ankyrins - metabolism ; Antigens, CD - genetics ; Antigens, CD - metabolism ; Biological and medical sciences ; Blood Proteins - deficiency ; Blood Proteins - genetics ; Carrier Proteins - genetics ; Carrier Proteins - metabolism ; CD47 Antigen ; Cytoskeletal Proteins ; Diseases of red blood cells ; Erythrocyte Membrane - metabolism ; Exons ; Glycophorins - metabolism ; Hematologic and hematopoietic diseases ; Humans ; Male ; Medical sciences ; Membrane Proteins ; Rh-Hr Blood-Group System - metabolism ; Spherocytosis, Hereditary - genetics ; Spherocytosis, Hereditary - metabolism
É parte de: Blood, 2002-09, Vol.100 (5), p.1878-1885
Notas: ObjectType-Case Study-2
SourceType-Scholarly Journals-1
ObjectType-Feature-4
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ObjectType-Report-1
ObjectType-Article-3
Descrição: We present data on a patient of South Asian origin with recessive hereditary spherocytosis (HS) due to absence of protein 4.2 [4.2 (−) HS]. Protein 4.2 cDNA sequence analysis showed the presence of a novel 41-bp frameshift deletion that predicts a truncated peptide designated protein 4.2 Hammersmith. Quantitative reverse transcription-polymerase chain reaction indicated that the mutant mRNA was unstable. Sequencing of protein 4.2 genomic DNA revealed that the deletion stems from aberrant splicing. The proband was homozygous for a G>T substitution at position 1747 (cDNA numbering) that activates a cryptic acceptor splice site within exon 11 of the protein 4.2 gene (EPB42). The proband's mother was found to be heterozygous for this substitution. Unlike protein 4.2 null mice, the proband's red cells showed no evidence for abnormal cation permeability. Quantitation of red cell membrane proteins was carried out by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE), Western blotting, and flow cytometric measurement. CD47, a protein associated with the Rh complex, was markedly reduced to about 1% (in the proband) and 65% (in the mother) that found in healthy controls. The Rh-associated glycoprotein migrated with a higher than normal apparent molecular weight on SDS-PAGE. There was no obvious reduction in Rh polypeptides. These observations indicate that protein 4.2 and CD47 interact in the human red cell membrane. They provide further evidence for an association between the band 3 complex (band 3, ankyrin, protein 4.2, glycophorin A) and the Rh complex (Rh-associated glycoprotein, Rh polypeptides, glycophorin B, CD47, LW) and define a point of attachment between the Rh complex and the red cell cytoskeleton.
Editor: Washington, DC: Elsevier Inc
Idioma: Inglês

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Absence of CD47 in protein 4.2–deficient hereditary spherocytosis in man: an interaction between the Rh complex and the band 3 complex

Bruce, Lesley J. ; Ghosh, Sandip ; King, May Jean ; Layton, D. Mark ; Mawby, William J. ; Stewart, Gordon W. ; Oldenborg, Per-Arne ; Delaunay, Jean ; Tanner, Michael J.A.

Washington, DC: Elsevier Inc

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