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Antigenic fractions from Taenia crassiceps metacestodes obtained by hydrophobicity for the immunodiagnosis of active and inactive forms of neurocysticercosis in human cerebrospinal fluid samples

da Silva, Gabriela B ; Nunes, Daniela S ; de Sousa, José Eduardo N ; Gonçalves-Pires, Maria do R.F ; Levenhagen, Marcelo A ; Costa-Cruz, Julia M

Parasitology international, 2017-04, Vol.66 (2), p.134-138 [Periódico revisado por pares]

Netherlands: Elsevier B.V

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Antigenic fractions from Taenia crassiceps metacestodes obtained by hydrophobicity for the immunodiagnosis of active and inactive forms of neurocysticercosis in human cerebrospinal fluid samples
Autor: da Silva, Gabriela B ; Nunes, Daniela S ; de Sousa, José Eduardo N ; Gonçalves-Pires, Maria do R.F ; Levenhagen, Marcelo A ; Costa-Cruz, Julia M
Assuntos: Animals ; Antibodies, Helminth - cerebrospinal fluid ; Antigens, Helminth - chemistry ; Antigens, Helminth - immunology ; Antigens, Helminth - isolation & purification ; Cerebrospinal fluid ; Chemical Fractionation - methods ; Diagnosis ; Enzyme-Linked Immunosorbent Assay ; Female ; Gastroenterology and Hepatology ; Humans ; Hydrophobic and Hydrophilic Interactions ; Immunoglobulin G - cerebrospinal fluid ; Infectious Disease ; Larva - chemistry ; Larva - immunology ; Male ; Mice ; Neurocysticercosis ; Neurocysticercosis - cerebrospinal fluid ; Neurocysticercosis - diagnosis ; Neurocysticercosis - immunology ; Polyethylene Glycols ; Predictive Value of Tests ; Sensitivity and Specificity ; Sodium Chloride ; Taenia - immunology ; Taenia - physiology ; Taenia crassiceps ; Triton X-114
É parte de: Parasitology international, 2017-04, Vol.66 (2), p.134-138
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Descrição: Abstract This study aimed to evaluate the total extract of Taenia crassiceps metacestodes (TC) and its antigenic fractions obtained by Triton X-114 fractionation techniques, such as detergent (DC) and aqueous (AC), in the immunodiagnosis of human neurocysticercosis (NCC). Cerebrospinal fluid samples were divided into two groups: Group 1 ( n = 40), which was further divided into active ( n = 20) and inactive ( n = 20) NCC, and Group 2 (control group), which comprised 39 CSF samples from patients who had another neurological disorder, were suffering from other infectious diseases of the brain or had other parasitic infections. The total extracts and antigenic fractions were tested by enzyme-linked immunosorbent assay (ELISA) to detect human IgG anti- Taenia solium. T. crassiceps fractions (DC and AC) showed the same value of sensitivity (Se), 100%, for active and inactive NCC and a specificity (Sp) of 97.4%. The DS fraction obtained from T. solium showed 100% Se for active NCC, 95% Se for inactive NCC and a 92.3% Sp. The AS fraction obtained from T. solium showed 100% Se for both active and inactive NCC and a 94.9% Sp. There was a positive correlation between the total saline extract of T. crassiceps (TC) and T. solium (TS) and their fractions (DC, AC, DS and AS). Positive predictive value, negative predictive value, diagnostic efficiency and Youden index were calculated. In conclusion, these results demonstrated that detergent and aqueous fractions obtained from T. crassiceps metacestodes are important sources of specific antigens and are efficient for immunodiagnosis of active and inactive NCC.
Editor: Netherlands: Elsevier B.V
Idioma: Inglês

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Antigenic fractions from Taenia crassiceps metacestodes obtained by hydrophobicity for the immunodiagnosis of active and inactive forms of neurocysticercosis in human cerebrospinal fluid samples

da Silva, Gabriela B ; Nunes, Daniela S ; de Sousa, José Eduardo N ; Gonçalves-Pires, Maria do R.F ; Levenhagen, Marcelo A ; Costa-Cruz, Julia M

Netherlands: Elsevier B.V

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