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Inhibition of the glucose and amino acid carriers of Lemna gibba by pretreatment with HgCl2

Golle, B ; Luttge, U

Physiologia plantarum, 1983, Vol.57 (1), p.62-66 [Periódico revisado por pares]

Oxford, UK: Blackwell Publishing Ltd

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  • Título:
    Inhibition of the glucose and amino acid carriers of Lemna gibba by pretreatment with HgCl2
  • Autor: Golle, B ; Luttge, U
  • Assuntos: Ecotoxicily ; mercuric chloride ; proton-cotransport ; SH-groups
  • É parte de: Physiologia plantarum, 1983, Vol.57 (1), p.62-66
  • Notas: ark:/67375/WNG-D4TZF03G-T
    istex:A537ECC583A765AE4E9C8FFA19AA881922972C65
    ArticleID:PPL62
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  • Descrição: The electrical resting potential across the plasmalemma of Lemna gibba L. (G 1) cells is −230 to −250 mV and the diffusion potential in the presence of 1 mol m−3 KCN + 1 mol m−3 salicylhydroxamic acid is about −100 mV. A concentration of 0.01 mol m−3 HgCl2 depolarises the transmembrane electrical potential in a largely reversible way. When the cells after 16 min of HgCl2‐application are returned to Hg‐free solution, the transmembrane electrical potential is only depolarised by 24 × 13 mV (SD, n = 13) compared with the potential prior to HgCl2 treatment. In contrast, a 16 min pretreatment with HgCl2 followed by a wash with mercury‐free solution reduces the transient depolarisations of transmembrane potential observed after addition of 5 mol m−3 D‐glncose or 1 mol m−3 L‐alaoine to about 60% of controls. These transient depolarisations are due to the onset of solute uptake. Accordingly, HgCl2‐pretreatment inhibits uptake of 14C‐3‐O‐methyl‐d‐glucose by more than 50% and uptake of 14C‐l‐alanine by more than 70%. Washing with 1 mol m−3 1,4‐dithiothreitol does not reverse this inhibition. It is, therefore, concluded that Hg2+ irreversibly binds to essential SH‐groups of the H+‐hexose and the H+‐amino‐acid cotransport carriers of Lemna gibba and inhibits these carriers without appreciably affecting the electrogenic proton‐extrusion pump.
  • Editor: Oxford, UK: Blackwell Publishing Ltd
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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