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Epidermal growth factor initiates DNA synthesis after a time-dependent sequence of regulatory events in Swiss 3T3 cells-interactions with hormones and growth factors

Otto, Angela M. ; Ulrich, Marie-Odile ; de Asua, Luis Jimenez

Journal of cellular physiology, 1981-08, Vol.108 (2), p.145-153 [Periódico revisado por pares]

Hoboken: Wiley Subscription Services, Inc., A Wiley Company

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Título:
Epidermal growth factor initiates DNA synthesis after a time-dependent sequence of regulatory events in Swiss 3T3 cells-interactions with hormones and growth factors
Autor: Otto, Angela M. ; Ulrich, Marie-Odile ; de Asua, Luis Jimenez
Assuntos: Animals ; Cells, Cultured ; DNA - biosynthesis ; Dose-Response Relationship, Drug ; Drug Synergism ; Epidermal Growth Factor - antagonists & inhibitors ; Epidermal Growth Factor - pharmacology ; Hydrocortisone - pharmacology ; In Vitro Techniques ; Insulin - pharmacology ; Mice ; Peptides - pharmacology ; Prostaglandins E - pharmacology ; Prostaglandins F - pharmacology ; Stimulation, Chemical ; Time Factors
É parte de: Journal of cellular physiology, 1981-08, Vol.108 (2), p.145-153
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ArticleID:JCP1041080205
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SourceType-Scholarly Journals-1
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Descrição: Epidermal growth factor (EGF) stimulates the initiation of DNA synthesis in Swiss 3T3 cells after a constant prereplicative period of 14–15 hours. The final rate of initiation follows apparent first‐order kinetics and can thus be quantified by a rate constant k. The value of k can be changed by later additions during the prereplicative period: When cells stimulated by a very low concentration of EGF, alone or with insulin, which results in a relatively low value of k, receive a saturating amount of EGF at 15 hours, then k is markedly increased after 4–6 hours. Insulin alone (up to 200 ng/ml) is unable to set the lag phase, but does have a synergistic effect on the value of k given by EGF. When added at 15 hours, insulin also increases k, but after a delay of 4–6 hours. In contrast, both hydrocortisone and prostaglandin E1 (PGE1) inhibit the stimulation of DNA synthesis by EGF only during the first 8 hours of the prereplicative period of decreasing the value of k. Prostaglandin F2α (PGF2α), which stimulates DNA synthesis in a similar mode as EGF, when added with EGF has a synergistic effect on DNA synthesis. This suggests that EGF and PGF2α, nevertheless, act through different regulatory events.
Editor: Hoboken: Wiley Subscription Services, Inc., A Wiley Company
Idioma: Inglês

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Epidermal growth factor initiates DNA synthesis after a time-dependent sequence of regulatory events in Swiss 3T3 cells-interactions with hormones and growth factors

Otto, Angela M. ; Ulrich, Marie-Odile ; de Asua, Luis Jimenez

Hoboken: Wiley Subscription Services, Inc., A Wiley Company

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