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MiR-320 inhibits the growth of glioma cells through downregulating PBX3

Pan, Cuicui ; Gao, Hua ; Zheng, Ni ; Gao, Qi ; Si, Yuanquan ; Zhao, Yueran

Biological research, 2017-09, Vol.50 (1), p.31-31, Article 31 [Periódico revisado por pares]

England: BioMed Central Ltd

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Título:
MiR-320 inhibits the growth of glioma cells through downregulating PBX3
Autor: Pan, Cuicui ; Gao, Hua ; Zheng, Ni ; Gao, Qi ; Si, Yuanquan ; Zhao, Yueran
Assuntos: Apoptosis ; BIOLOGY ; Brain Neoplasms - metabolism ; Brain Neoplasms - pathology ; Care and treatment ; Cell Differentiation - drug effects ; Cell Line, Tumor ; Cell Proliferation - drug effects ; Down-Regulation ; Gene Expression Regulation, Neoplastic ; Genetic aspects ; Glioma ; Glioma - metabolism ; Glioma - pathology ; Gliomas ; Growth ; Health aspects ; Homeodomain Proteins - metabolism ; Humans ; Luciferase reporter assay ; MicroRNA ; MicroRNAs - metabolism ; MiR-320 ; PBX3 ; Physiological aspects ; Proto-Oncogene Proteins - metabolism ; Up-Regulation
É parte de: Biological research, 2017-09, Vol.50 (1), p.31-31, Article 31
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: MiR-320 is downregulated in multiple cancers, including glioma and acts as tumor suppressor through inhibiting tumor cells proliferation and inducing apoptosis. PBX3 (Pre-B cell leukemia homeobox 3), a putative target gene of miR-320, has been reported to be upregulated in various tumors and promote tumor cell growth through regulating MAKP/ERK pathway. This study aimed to verify whether miR-320 influences glioma cells growth through regulating PBX3. Twenty-four human glioma and paired adjacent nontumorous tissues were collected for determination of miR-320 and PBX3 expression using RT-qPCR and western blot assays. Luciferase reporter assay was performed to verify the interaction between miR-320 and its targeting sequence in the 3' UTR of PBX3 in glioma cells U87 and U251. Increased miR-320 level in U87 and U251 cells was achieved through miR-320 mimic transfection and the effect of which on glioma cells growth, proliferation, cell cycle, apoptosis and activation of Raf-1/MAPK pathway was determined using MTT, colony formation, flow cytometry and western blot assays. PBX3 knockdown was performed using shPBX3 and the influence on MAPK pathway activation was evaluated. MiR-320 downregulation and PBX3 upregulation was found in glioma tissues. Luciferase reporter assays identified miR-320 directly blinds to the 3' UTR of PBX3 in glioma cells. MiR-320 mimic transfection suppressed glioma cells proliferation, and induced cell cycle arrest and apoptosis. Both miR-320 overexpression and PBX3 knockdown inhibited Raf-1/MAPK activation. MiR-320 may suppress glioma cells growth and induced apoptosis through the PBX3/Raf-1/MAPK axis, and miR-320 oligonucleotides may be a potential cancer therapeutic for glioma.
Editor: England: BioMed Central Ltd
Idioma: Inglês;Português

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MiR-320 inhibits the growth of glioma cells through downregulating PBX3

Pan, Cuicui ; Gao, Hua ; Zheng, Ni ; Gao, Qi ; Si, Yuanquan ; Zhao, Yueran

England: BioMed Central Ltd

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