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Immunolocalization of antioxidant enzymes in testis of rams submitted to long-term heat stress

Hamilton, Thais Rose dos Santos ; Duarte, Gabriela Esteves ; Visintin, José Antonio ; Assumpção, Mayra Elena Ortiz D’Ávila

Zygote (Cambridge), 2019-12, Vol.27 (6), p.432-435 [Periódico revisado por pares]

Cambridge, UK: Cambridge University Press

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Título:
Immunolocalization of antioxidant enzymes in testis of rams submitted to long-term heat stress
Autor: Hamilton, Thais Rose dos Santos ; Duarte, Gabriela Esteves ; Visintin, José Antonio ; Assumpção, Mayra Elena Ortiz D’Ávila
Assuntos: Animals ; Antioxidants ; Antioxidants - metabolism ; Catalysis ; Deoxyribonucleic acid ; DNA ; DNA damage ; Enzymes ; Glutathione ; Glutathione peroxidase ; Glutathione Peroxidase - metabolism ; Glutathione reductase ; Glutathione Reductase - metabolism ; Heat ; Heat stress ; Heat tolerance ; Heat-Shock Response - physiology ; Hypoxia ; Immunohistochemistry ; Immunohistochemistry - methods ; Insulation ; Male ; Motility ; Orchiectomy ; Oxidative stress ; Oxidative Stress - physiology ; Peroxidase ; Polyclonal antibodies ; Rams ; Reductases ; Seminiferous tubule ; Seminiferous Tubules - cytology ; Seminiferous Tubules - enzymology ; Sheep ; Short Communication ; Software ; Sperm ; Spermatids - cytology ; Spermatids - enzymology ; Spermatocytes - cytology ; Spermatocytes - enzymology ; Spermatogenesis ; Spermatogonia - cytology ; Spermatogonia - enzymology ; Statistical analysis ; Superoxide dismutase ; Superoxide Dismutase - metabolism ; Testes ; Testis - cytology ; Testis - enzymology ; Time Factors
É parte de: Zygote (Cambridge), 2019-12, Vol.27 (6), p.432-435
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SourceType-Scholarly Journals-1
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Descrição: Long-term heat stress (HS) induced by testicular insulation generates oxidative stress (OS) on the testicular environment; consequently activating antioxidant enzymes such as superoxide dismutase (SOD), glutathione reductase (GR) and glutathione peroxidase (GPx). The aim of this work was to immunolocalize antioxidant enzymes present in different cells within the seminiferous tubule when rams were submitted to HS. Rams were divided into control (n = 6) and treated group (n = 6), comprising rams subjected to testicular insulation for 240 h. After the testicular insulation period, rams were subjected to orchiectomy. Testicular fragments were submitted to immunohistochemistry for staining against SOD, GR and GPx enzymes. We observed immunolocalization of GPx in more cell types of the testis after HS and when compared with other enzymes. In conclusion, GPx is the main antioxidant enzyme identified in testicular cells in an attempt to maintain oxidative balance when HS occurs.
Editor: Cambridge, UK: Cambridge University Press
Idioma: Inglês

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Immunolocalization of antioxidant enzymes in testis of rams submitted to long-term heat stress

Hamilton, Thais Rose dos Santos ; Duarte, Gabriela Esteves ; Visintin, José Antonio ; Assumpção, Mayra Elena Ortiz D’Ávila

Cambridge, UK: Cambridge University Press

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