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Development of a magnetically stabilized fluidized bed bioreactor for enzymatic synthesis of 2-ethylhexyl oleate

Mateus Vinicius Casagrande da Silva Amanda Bahia de Souza Rangel; Cíntia M. R Rosa; Gabrielle Policarpo de Assis; Leandro Gonçalves de Aguiar; Larissa Freitas

Bioprocess and biosystems engineering (internet) v.46, p.1665-1676, 2023

Garching, Gw Springer 2023

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  • Título:
    Development of a magnetically stabilized fluidized bed bioreactor for enzymatic synthesis of 2-ethylhexyl oleate
  • Autor: Mateus Vinicius Casagrande da Silva
  • Amanda Bahia de Souza Rangel; Cíntia M. R Rosa; Gabrielle Policarpo de Assis; Leandro Gonçalves de Aguiar; Larissa Freitas
  • Assuntos: BIOTECNOLOGIA; Computer Simulation; Esterifcation; Immobilized Lipase; Magnetic Polymer Support; Magnetically Stabilized Fuidized Bed Bioreactor
  • É parte de: Bioprocess and biosystems engineering (internet) v.46, p.1665-1676, 2023
  • Notas: Disponível em: https://doi.org/10.1007/s00449-023-02928-8. Acesso em: 23 nov. 2023
  • Descrição: This study aimed to develop and investigate the synthesis of 2-ethylhexyl oleate catalyzed by Candida antarctica lipase immobilized on magnetic poly(styrene-co-divinylbenzene) (STY–DVB-M) particles in a magnetically stabilized fuidized bed reactor (MSFBR) operated in continuous mode. The physical properties of the copolymer were characterized by Fouriertransform infrared spectroscopy (FTIR), diferential scanning calorimetry (DSC), and thermogravimetric analysis (TGA). The glass transition temperature was 85.68 °C, and the onset of thermal degradation occurred at 406.66 °C. Syntheses were performed at 50 °C using a space time of 12 h and a bed porosity of 0.892. Assays were conducted to assess the infuence of magnetic feld intensity (5 to 15 mT) on reaction yield, ester concentration, and productivity. The highest productivity was 0.850±0.023 mmol g−1 h−1, obtained with a magnetic feld intensity of 15 mT. An operational stability test was performed under these conditions, revealing a biocatalyst half-life of 2148 h (179 operation cycles) and a thermal deactivation constant of 3.23× 10−4 h−1 (R2=0.9446). Computational simulations and mathematical modeling were performed using Scilab based on ping-pong bi–bi kinetics and molar balances of reaction species. The model provided consistent results of interstitial velocity and good prediction of reaction yields, with R2=0.926. These fndings demonstrate that the studied technique can provide improvements in biocatalytic processes, representing a promising strategy for the enzymatic synthesis of 2-ethylhexyl oleate.
  • Editor: Garching, Gw Springer
  • Data de criação/publicação: 2023
  • Formato: p. 1665-1676.
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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