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Ion exchange chromatography of glucosamine and galactosamine in microgram amounts with quantitative determination and specific radioactivity assay

Lohmander, Stefan

Biochimica et biophysica acta. General subjects, 1972-05, Vol.264 (3), p.411-417 [Periódico revisado por pares]

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Título:
Ion exchange chromatography of glucosamine and galactosamine in microgram amounts with quantitative determination and specific radioactivity assay
Autor: Lohmander, Stefan
Assuntos: Clinical Medicine ; Klinisk medicin ; Medical and Health Sciences ; Medicin och hälsovetenskap ; Orthopedics ; Ortopedi
É parte de: Biochimica et biophysica acta. General subjects, 1972-05, Vol.264 (3), p.411-417
Descrição: A rapid method for the separation and quantitative determination, including radioassay, of glucosamine and galactosamine in microgram amounts is described. It is based upon the use of a column of Aminex A-5 ion exchange resin eluted with a phosphate buffer at pH 7.00 and 60 °C. From each fraction half the volume is used for a scaled down Elson-Morgan procedure and other half for liquid scintillation counting. Amounts of about 0.01–1 μmole of hexosamine may be separated and determined.
Editor: Elsevier B.V
Idioma: Inglês

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