Idioma:

Disruption of the Chitin Biosynthetic Pathway Results in Significant Changes in the Cell Growth Phenotypes and Biosynthesis of Secondary Metabolites of Monascus purpureus

Shu, Meng ; Lu, Pengxin ; Liu, Shuai ; Zhang, Song ; Gong, Zihan ; Cai, Xinru ; Zhou, Bo ; Lin, Qinlu ; Liu, Jun

Journal of fungi (Basel), 2022-08, Vol.8 (9), p.910 [Periódico revisado por pares]

Basel: MDPI AG

Texto completo disponível

Citações Citado por

Enviar para

Título:
Disruption of the Chitin Biosynthetic Pathway Results in Significant Changes in the Cell Growth Phenotypes and Biosynthesis of Secondary Metabolites of Monascus purpureus
Autor: Shu, Meng ; Lu, Pengxin ; Liu, Shuai ; Zhang, Song ; Gong, Zihan ; Cai, Xinru ; Zhou, Bo ; Lin, Qinlu ; Liu, Jun
Assuntos: Biosynthesis ; Cell growth ; Chitin ; Chitin synthase ; Citrinin ; Cloning ; Digital cameras ; Environmental factors ; Enzymes ; Fermentation ; Gene expression ; Genes ; Genetic engineering ; Germination ; Hyphae ; MAP kinase ; Metabolism ; Metabolites ; Monascus ; Monascus purpureus ; Morphology ; Mycelia ; Phenotypes ; Pigments ; Plasmids ; Regulation ; secondary metabolite ; Secondary metabolites ; Signal transduction ; Spores ; stress tolerance ; Transcriptomes
É parte de: Journal of fungi (Basel), 2022-08, Vol.8 (9), p.910
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
These authors contributed equally to this work.
Descrição: In this study, the gene monascus-5162 from Monascus purpureus LQ-6, identified as chitin synthase gene VI (chs6), was knocked out to disrupt the chitin biosynthetic pathway and regulate the biosynthesis of Monascus pigments (MPs) and citrinin. The results showed that the aerial hyphae on a solid medium were short and sparse after the deletion of chs6 in M. purpureus LQ-6, significantly reducing the germination percentage of active spores to approximately 22%, but the colony diameter was almost unaffected. Additionally, the deletion of chs6 changed the mycelial morphology of M. purpureus LQ-6 during submerged fermentation and increased its sensitivity to environmental factors. MP and citrinin biosynthesis was dramatically inhibited in the recombinant strain. Furthermore, comparative transcriptome analysis revealed that the pathways related to spore development and growth, including the MAPK signaling pathway, chitin biosynthetic pathway, and regulatory factors LaeA and WetA genes, were significantly downregulated in the early phase of fermentation. The mRNA expression levels of genes in the cluster of secondary metabolites were significantly downregulated, especially those related to citrinin biosynthesis. This is the first detailed study to reveal that chs6 plays a vital role in regulating the cell growth and secondary metabolism of the Monascus genus.
Editor: Basel: MDPI AG
Idioma: Inglês

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

Disruption of the Chitin Biosynthetic Pathway Results in Significant Changes in the Cell Growth Phenotypes and Biosynthesis of Secondary Metabolites of Monascus purpureus

Shu, Meng ; Lu, Pengxin ; Liu, Shuai ; Zhang, Song ; Gong, Zihan ; Cai, Xinru ; Zhou, Bo ; Lin, Qinlu ; Liu, Jun

Basel: MDPI AG

Buscando em bases de dados remotas. Favor aguardar.