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LIP19, a basic region leucine zipper protein, is a fos-like molecular switch in the cold signaling of rice [Oryza sativa] plants

Shimizu, H.(Tohoku Univ., Sendai (Japan)) ; Sato, K ; Berberich, T ; Miyazaki, A ; Ozaki, R ; Imai, R ; Kusano, T

Plant and cell physiology, 2005-10, Vol.46 (10), p.1623-1634 [Periódico revisado por pares]

Japan: Oxford University Press

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Título:
LIP19, a basic region leucine zipper protein, is a fos-like molecular switch in the cold signaling of rice [Oryza sativa] plants
Autor: Shimizu, H.(Tohoku Univ., Sendai (Japan)) ; Sato, K ; Berberich, T ; Miyazaki, A ; Ozaki, R ; Imai, R ; Kusano, T
Assuntos: ADN ; Amino Acid Sequence ; amino acid sequences ; Base Sequence ; basic region/leucine zipper ; Basic-Leucine Zipper Transcription Factors - chemistry ; Basic-Leucine Zipper Transcription Factors - metabolism ; Basic-Leucine Zipper Transcription Factors - physiology ; BiFC ; bimolecular fluorescence complementation ; binding form ; BIOLOGIA MOLECULAR ; BIOLOGIE MOLECULAIRE ; bZIP ; bZIP protein ; COLD ; Cold signaling ; cold stress ; Cold Temperature ; complementary DNA ; DIG ; digoxigenin ; Dimerization ; dithiothreitol ; DNA ; DNA binding ; DNA Primers ; DNA, Plant - metabolism ; DNA-binding proteins ; DTT ; Electrophoretic Mobility Shift Assay ; EMSA ; FRIO ; FROID ; GFP ; glutathione S-transferase ; green fluorescent protein ; GST ; Heterodimer ; IPTG ; isopropyl-β-d-thiogalactopyranoside ; leaf blade ; leucine zipper ; Leucine Zippers ; MOLECULAR BIOLOGY ; Molecular Sequence Data ; Molecular switch ; nucleotide sequences ; o-nitrophenyl-β-d-galactopyranoside ; OBF1 ; ocs ; ocs-element Binding Factor 1 ; octopine synthase ; ONPG ; open reading frame ; ORF ; Oryza - metabolism ; ORYZA SATIVA ; Oryza sativa OBF1 ; OsOBF1 ; phenylmethylsulfonyl fluoride ; plant biochemistry ; plant proteins ; Plant Proteins - chemistry ; Plant Proteins - metabolism ; Plant Proteins - physiology ; plant response ; PMSF ; protein-protein interactions ; Rice ; Saccharomyces cerevisiae ; seedlings ; Sequence Homology, Amino Acid ; Signal Transduction ; synthetic drop-out ; tissue distribution ; transcription factor ; two hybrid system techniques ; yellow fluorescent protein ; YFP
É parte de: Plant and cell physiology, 2005-10, Vol.46 (10), p.1623-1634
Notas: 2006004814
F40
Corresponding author: E-mail, kusano@ige.tohoku.ac.jp; Fax, +81-22-217-5709.
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Descrição: The rice low-temperature-induced lip19 gene encodes a 148-amino-acid basic region/leucine zipper (bZIP) protein, termed LIP19. In this study we characterized LIP19 and showed that it lacks the usual ability of bZIP proteins to homodimerize and to bind DNA, as does the Fos protein in mammals. Using a yeast two-hybrid system, the cDNA clones whose products interact with LIP19 were screened. This search revealed a clone termed OsOBF1 (Oryza sativa OBF1) that encodes a new bZIP protein (OsOBF1). This protein forms a homodimer and binds to the hexamer motif sequence (5'-ACGTCA-3'). The protein-protein interaction in homo- and hetero-combinations between LIP19 and OsOBF1 was confirmed in vitro and in planta. LIP19 and OsOBF1 most likely interact with each other more strongly than OsOBF1 interacts with itself, and the resulting heterodimer binds to the C/G hybrid sequence but not to the hexamer sequence. Whereas the expression patterns of LIP19 and OsOBF1 in response to low temperatures were totally opposite, the locations of their expression were almost identical. Based upon the presented data, we propose a model describing the low-temperature signal switching mediated by LIP19 in rice.
Editor: Japan: Oxford University Press
Idioma: Inglês

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LIP19, a basic region leucine zipper protein, is a fos-like molecular switch in the cold signaling of rice [Oryza sativa] plants

Shimizu, H.(Tohoku Univ., Sendai (Japan)) ; Sato, K ; Berberich, T ; Miyazaki, A ; Ozaki, R ; Imai, R ; Kusano, T

Japan: Oxford University Press

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