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Mating activates the heme peroxidase HPX15 in the sperm storage organ to ensure fertility in Anopheles gambiae

Shaw, W. Robert ; Teodori, Eleonora ; Mitchell, Sara N. ; Baldini, Francesco ; Gabrieli, Paolo ; Rogers, David W. ; Catterucci, Flaminia

Proceedings of the National Academy of Sciences - PNAS, 2014-04, Vol.111 (16), p.5854-5859 [Periódico revisado por pares]

United States: National Academy of Sciences

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Mating activates the heme peroxidase HPX15 in the sperm storage organ to ensure fertility in Anopheles gambiae
Autor: Shaw, W. Robert ; Teodori, Eleonora ; Mitchell, Sara N. ; Baldini, Francesco ; Gabrieli, Paolo ; Rogers, David W. ; Catterucci, Flaminia
Assuntos: Animal reproduction ; Animal Structures - cytology ; Animal Structures - drug effects ; Animal Structures - enzymology ; Animal Structures - ultrastructure ; Animals ; Anopheles - drug effects ; Anopheles - enzymology ; Anopheles - genetics ; Biological Sciences ; Drosophila ; Ecdysone - pharmacology ; Enzyme Activation - drug effects ; Enzymes ; Epithelial cells ; Female ; Female animals ; Fertility - drug effects ; Fertility - genetics ; Gene expression regulation ; Gene Expression Regulation - drug effects ; Heme - metabolism ; Insect Proteins - genetics ; Insect Proteins - metabolism ; Insecticides ; Malaria ; Male ; Mating behavior ; Mosquitoes ; Mosquitos ; Ova ; Peroxidase - genetics ; Peroxidase - metabolism ; Resistance to control ; Reverse Transcriptase Polymerase Chain Reaction ; Secretory cells ; Sexual Behavior, Animal - drug effects ; Spermatheca ; Spermatozoa ; Spermatozoa - cytology ; Spermatozoa - drug effects ; Spermatozoa - enzymology ; Spermatozoa - ultrastructure ; Transcription, Genetic - drug effects
É parte de: Proceedings of the National Academy of Sciences - PNAS, 2014-04, Vol.111 (16), p.5854-5859
Notas: http://dx.doi.org/10.1073/pnas.1401715111
Author contributions: W.R.S., E.T., F.B., P.G., D.W.R., and F.C. designed research; W.R.S., E.T., F.B., P.G., and D.W.R. performed research; W.R.S., E.T., S.N.M., F.B., P.G., and F.C. analyzed data; and W.R.S., E.T., and F.C. wrote the paper.
2Present address: Institute of Biodiversity, Animal Health and Comparative Medicine, University of Glasgow, Glasgow G12 8QQ, United Kingdom.
1W.R.S. and E.T. contributed equally to this work.
3Present address: Max Planck Research Group for Experimental Evolution, Max Planck Institute for Evolutionary Biology, 24306 Plön, Germany.
Edited by Anthony A. James, University of California, Irvine, CA, and approved March 12, 2014 (received for review January 28, 2014)
Descrição: Anopheles gambiae mosquitoes are major African vectors of malaria, a disease that kills more than 600,000 people every year. Given the spread of insecticide resistance in natural mosquito populations, alternative vector control strategies aimed at reducing the reproductive success of mosquitoes are being promoted. Unlike many other insects, An. gambiae females mate a single time in their lives and must use sperm stored in the sperm storage organ, the spermatheca, to fertilize a lifetime's supply of eggs. Maintenance of sperm viability during storage is therefore crucial to the reproductive capacity of these mosquitoes. However, to date, no information is available on the factors and mechanisms ensuring sperm functionality in the spermatheca. Here we identify cellular components and molecular mechanisms used by An. gambiae females to maximize their fertility. Pathways of energy metabolism, cellular transport, and oxidative stress are strongly regulated by mating in the spermatheca. We identify the mating-induced heme peroxidase (HPX) 15 as an important factor in long-term fertility, and demonstrate that its function is required during multiple gonotrophic cycles. We find that HPX15 induction is regulated by sexually transferred 20-hydroxy-ecdysone (20E), a steroid hormone that is produced by the male accessory glands and transferred during copulation, and that expression of this peroxidase is mediated via the 20E nuclear receptor. To our knowledge, our findings provide the first evidence of the mechanisms regulating fertility in Anopheles , and identify HPX15 as a target for vector control.
Editor: United States: National Academy of Sciences
Idioma: Inglês

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Mating activates the heme peroxidase HPX15 in the sperm storage organ to ensure fertility in Anopheles gambiae

Shaw, W. Robert ; Teodori, Eleonora ; Mitchell, Sara N. ; Baldini, Francesco ; Gabrieli, Paolo ; Rogers, David W. ; Catterucci, Flaminia

United States: National Academy of Sciences

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